Time lapse images of cytoskeleton and cordon bleu, a microtubule-associated protein
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Tsukita K, et. al. Sci Adv. 2023 Feb 15;9(7):eadf6358. / Tsukita K, Kitamata M, Kashihara H, Yano T, Fujiwara I, Day TF, Katsuno T, Kim J, Takenaga F, Tanaka H, Park S, Miyata M, Watanabe H, Kondoh G, Takahashi R, Tamura A, Tsukita S. Phase separation of an actin nucleator by junctional microtubules regulates epithelial function. Sci Adv. 2023 Feb 15;9(7):eadf6358.
Bioluminescent time-lapse imaging of Ascl1-Luciferse reporter mice's brain slices after iPaD virus injection
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Kaise T, et. al. Genes Dev. 2022 Jan 1;36(1-2):23-37 / Kaise T, Fukui M, Sueda R, Piao W, Yamada M, Kobayashi T, Imayoshi I, Kageyama R. Functional rejuvenation of aged neural stem cells by Plagl2 and anti-Dyrk1a activity. Genes Dev. 2022 Jan 1;36(1-2):23-37
Ohgushi M, et. al. Cell Rep. 2022 Jun 21;39(12):110973 / Delamination of trophoblast-like syncytia from the amniotic ectodermal analogue in human primed embryonic stem cell-based differentiation model. Ohgushi M, Taniyama N, Vandenbon A, Eiraku M. Cell Rep. 2022 Jun 21;39(12):110973
MCF-7 cells stained by 12 different colors of Rdots
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Nishiyama R et. al., PNAS Nexus. 2023 Jan 14;2(2):pgad001. / Nishiyama R, Hiramatsu K, Kawamura S, Dodo K, Furuya K, de Pablo JG, Takizawa S, Min W, Sodeoka M, Goda K. Color-scalable flow cytometry with Raman tags. PNAS Nexus. 2023 Jan 14;2(2):pgad001.
Raman spectral data of mouse fixed tissues and live HeLa cells using multiline illumination.
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Mochizuki K, et. al. Biomed Opt Express. 2023 Feb 7;14(3):1015-1026. / Mochizuki K, Kumamoto Y, Maeda S, Tanuma M, Kasai A, Takemura M, Harada Y, Hashimoto H, Tanaka H, Smith NI, Fujita K. High-throughput line-illumination Raman microscopy with multislit detection. Biomed Opt Express. 2023 Feb 7;14(3):1015-1026.
Detection of multiple enzyme activities simultaneously in live cells by using activatable Raman probes
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Fujioka H, et. al. J Am Chem Soc. 2023 Apr 26;145(16):8871-8881. / Activatable Raman Probes Utilizing Enzyme-Induced Aggregate Formation for Selective Ex Vivo Imaging.
Fujioka H, Kawatani M, Spratt SJ, Komazawa A, Misawa Y, Shou J, Mizuguchi T, Kosakamoto H, Kojima R, Urano Y, Obata F, Ozeki Y, Kamiya M. J Am Chem Soc. 2023 Apr 26;145(16):8871-8881.
Super-resolution vibrational imaging based on photoswitchable Raman probe
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Shou J, et. al. Sci Adv. 2023 Jun 16;9(24):eade9118. / Shou J, Komazawa A, Wachi Y, Kawatani M, Fujioka H, Spratt SJ, Mizuguchi T, Oguchi K, Akaboshi H, Obata F, Tachibana R, Yasunaga S, Mita Y, Misawa Y, Kojima R, Urano Y, Kamiya M, Ozeki Y. Sci Adv. 2023 Jun 16;9(24):eade9118.
Intracellular distribution of exogenous H-Ras in HeLa cells.
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Ikari M, et. al. JACS Au. 2023 Jun 1;3(6):1658-1669. / Ikari M, Yagi H, Kasai T, Inomata K, Ito M, Higuchi K, Matsuda N, Ito Y, Kigawa T. Direct Observation of Membrane-Associated H-Ras in the Native Cellular Environment by In-Cell 19F-NMR Spectroscopy. JACS Au. 2023 Jun 1;3(6):1658-1669.
Coordinate normalization of Arabidopsis zygote growth.
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Kang Z, et. al. Plant Cell Physiol. 2023 Dec 6;64(11):1279-1288. / Kang Z, Matsumoto H, Nonoyama T, Nakagawa S, Ishimoto Y, Tsugawa S, Ueda M. Coordinate Normalization of Live-Cell Imaging Data Reveals Growth Dynamics of the Arabidopsis Zygote. Plant Cell Physiol. 2023 Dec 6;64(11):1279-1288.
Inhibition of SARS-CoV-2 virus infection to human airway organoids by autophagy-related compounds.
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Hashimoto R, et. al. Mol Pharm. 2023 Apr 3;20(4):2276-2287. / Hashimoto R, Tamura T, Watanabe Y, Sakamoto A, Yasuhara N, Ito H, Nakano M, Fuse H, Ohta A, Noda T, Matsumura Y, Nagao M, Yamamoto T, Fukuhara T, Takayama K. Evaluation of Broad Anti-Coronavirus Activity of Autophagy-Related Compounds Using Human Airway Organoids. Mol Pharm. 2023 Apr 3;20(4):2276-2287.
Time-lapse images of cell dynamics in different E. coli strain cells after gene deletion by blue-light illumination.
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Koganezawa Y, et. al. (2022) Elife, May 10;11:e74486. / Koganezawa Y, Umetani M, Sato M, Wakamoto Y. History-dependent physiological adaptation to lethal genetic modification under antibiotic exposure. Elife. 2022 May 10;11:e74486.
Confocal immunohistochemistry images and BDML file for quantitative information of SMA/laminin α2 mouse vessel-type classification for machine learning.
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Oishi M, Passlick S, Yamazaki Y, Unekawa M, Adachi R, Yamada M, Imayoshi I, Abe Y, Steinhäuser C, Tanaka KF. Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter. Glia. 2023 Feb;71(2):317-333.
Confocal immunohistochemistry images and BDML file for quantitative information of SMA/laminin α2 mouse vessel-type classification for machine learning.
Paper info
Oishi M, et. al. (2023) Glia. Feb;71(2):317-333. / Oishi M, Passlick S, Yamazaki Y, Unekawa M, Adachi R, Yamada M, Imayoshi I, Abe Y, Steinhäuser C, Tanaka KF. Separate optogenetic manipulation of Nerve/glial antigen 2 (NG2) glia and mural cells using the NG2 promoter. Glia. 2023 Feb;71(2):317-333.
Cryo-electron microscopy (cryo-EM) microscopy images of human ribosomes isolated from METTL18 KO cells.
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Matsuura-Suzuki E, et. al. Elife. 2022 Jun 8;11:e72780. / Matsuura-Suzuki E, Shimazu T, Takahashi M, Kotoshiba K, Suzuki T, Kashiwagi K, Sohtome Y, Akakabe M, Sodeoka M, Dohmae N, Ito T, Shinkai Y, Iwasaki S. METTL18-mediated histidine methylation of RPL3 modulates translation elongation for proteostasis maintenance. Elife. 2022 Jun 8;11:e72780.
Time-lapse images of L1210 cells growth and division in the mammalian mother machine microfluidic device.
Paper info
Seita, A et. al. (2021) PLoS One 16, e0236534. / Seita, A., Nakaoka, H., Okura, R., and Wakamoto, Y. (2021). Intrinsic growth heterogeneity of mouse leukemia cells underlies differential susceptibility to a growth-inhibiting anticancer drug. PLoS One 16, e0236534. 10.1371/journal.pone.0236534.
Time-lapse or z-stack images of EGFR signaling dynamics in epithelial homeostasis of Drosophila embyos.
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Yoshida K, et. al. Development. 2023 Mar 1;150(5):dev201231. / Yoshida K, Hayashi S. Epidermal growth factor receptor signaling protects epithelia from morphogenetic instability and tissue damage in Drosophila. Development. 2023 Mar 1;150(5):dev201231.
Time-course fulorescence images of dynamic changes in histone H3K9me3 in living cells.
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Sasaki K, Suzuki M, Sonoda T, Schneider-Poetsch T, Ito A, Takagi M, Fujishiro S, Sohtome Y, Dodo K, Umehara T, Aburatani H, Shin-Ya K, Nakao Y, Sodeoka M, Yoshida M. Visualization of the dynamic interaction between nucleosomal histone H3K9 tri-methylation and HP1α chromodomain in living cells. Cell Chem Biol. 2022 Jul 21;29(7):1153-1161.e5.
Time-course images of HeLa cells dynamics with fluorescence proteins vgCam and ATeam1.03.
Paper info
Sugiura K, et. al. (2022) Commun Biol. Nov 3;5(1):1172. / Sugiura K, Nagai T. Extension of the short wavelength side of fluorescent proteins using hydrated chromophores, and its application. Commun Biol. 2022 Nov 3;5(1):1172.
Time-course fulorescence images of dynamic changes in histone H3K9me3 in living cells.
Paper info
Sasaki K, et. al. (2022) Cell Chem Biol, Jul 21;29(7):1153-1161.e5. / Sasaki K, Suzuki M, Sonoda T, Schneider-Poetsch T, Ito A, Takagi M, Fujishiro S, Sohtome Y, Dodo K, Umehara T, Aburatani H, Shin-Ya K, Nakao Y, Sodeoka M, Yoshida M. Visualization of the dynamic interaction between nucleosomal histone H3K9 tri-methylation and HP1α chromodomain in living cells. Cell Chem Biol. 2022 Jul 21;29(7):1153-1161.e5.
Live imaging of caspase cascades activity in apoptosis-induced HeLa cells.
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Suzuki M, et. al. Sci Rep. 2022 Dec 7;12(1):21160. / Suzuki M, Shindo Y, Yamanaka R, Oka K. Live imaging of apoptotic signaling flow using tunable combinatorial FRET-based bioprobes for cell population analysis of caspase cascades. Sci Rep. 2022 Dec 7;12(1):21160.
Time-lapse imaging of reversible intercellular NCad interactions by INCIDER in HEK293T cells.
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Kanadome T, et. al. (2022) Commun Biol. Oct 7;5(1):1065. / Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, Matsuda T. Development of intensiometric indicators for visualizing N-cadherin interaction across cells. Commun Biol. 2022 Oct 7;5(1):1065.
Fluorescence images of immunostaining analysis of SARS-CoV-2 NP in hepatocytes of bile duct (ibd-LoC) or blood vessel (bv-LoC) at 2 or 6 days post-infection (dpi).
Paper info
Deguchi S, et. al. (2023) PNAS Nexus, Mar 7;2(3):pgad029. / Deguchi S, Kosugi K, Hashimoto R, Sakamoto A, Yamamoto M, Krol RP, Gee P, Negoro R, Noda T, Yamamoto T, Torisawa YS, Nagao M, Takayama K. Elucidation of the liver pathophysiology of COVID-19 patients using liver-on-a-chips. PNAS Nexus. 2023 Mar 7;2(3):pgad029.
Time-lapse FRAP assay images of DNAPTP6 dynamics in cells or not
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Asamitsu S, et. al. Sci Adv. 2023 Feb 24;9(8):eade2035. / Asamitsu S, Yabuki Y, Matsuo K, Kawasaki M, Hirose Y, Kashiwazaki G, Chandran A, Bando T, Wang DO, Sugiyama H, Shioda N. RNA G-quadruplex organizes stress granule assembly through DNAPTP6 in neurons. Sci Adv. 2023 Feb 24;9(8):eade2035.
Images of immotile cilia dynamics in wild-type mouse embryo or iv/iv mutant embryo
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Katoh TA, et. al. Science. 2023 Jan 6;379(6627):66-71. / Katoh TA, Omori T, Mizuno K, Sai X, Minegishi K, Ikawa Y, Nishimura H, Itabashi T, Kajikawa E, Hiver S, Iwane AH, Ishikawa T, Okada Y, Nishizaka T, Hamada H. Immotile cilia mechanically sense the direction of fluid flow for left-right determination. Science. 2023 Jan 6;379(6627):66-71.
Observation of dynamic mitochondrial distribution during development
and asymmetric division of rice zygotes
Paper info
Aini H, Sato Y, Uno K, Higashiyama T, Okamoto T. Dynamics of mitochondrial distribution during development and asymmetric division of rice zygotes. Plant Reprod. 2022 Mar;35(1):47-60.
Fluorescence images or time-lapse images of mitochondrial distribution dynamics during the development of rice zygotes
Paper info
Aini H, et. al. (2022) Plant Reprod. Mar;35(1):47-60. / Aini H, Sato Y, Uno K, Higashiyama T, Okamoto T. Dynamics of mitochondrial distribution during development and asymmetric division of rice zygotes. Plant Reprod. 2022 Mar;35(1):47-60.
Time-series images of behavioral activity of wild-type or mutant C. elegans in fed or starved situation.
Paper info
Arata Y, et. al. (2022) Sci Rep. 2022 Jun 21;12(1):10481. / Arata Y, Shiga I, Ikeda Y, Jurica P, Kimura H, Kiyono K, Sako Y. Insulin signaling shapes fractal scaling of C. elegans behavior. Sci Rep. 2022 Jun 21;12(1):10481.
Time-series images of daily patterns of wake, NREM, and REM sleep in control or CRF-Cre/Bmal1flox/flox mice under LD and DD.
Paper info
Hung CJ, et. al. (2022) Front Neurosci, 2022 Feb 18;15:808754. / Hung CJ, Yamanaka A, Ono D. Conditional Knockout of Bmal1 in Corticotropin-Releasing Factor Neurons Does Not Alter Sleep-Wake Rhythm in Mice. Front Neurosci. 2022 Feb 18;15:808754.
Time-lapse imaging of hba reporter expression in the GFP knock-in N. furzeri embryos.
Paper info
Oginuma M, et. al. (2022) Sci Rep, Jul 8;12(1):11628. / Oginuma M, Nishida M, Ohmura-Adachi T, Abe K, Ogamino S, Mogi C, Matsui H, Ishitani T. Rapid reverse genetics systems for Nothobranchius furzeri, a suitable model organism to study vertebrate aging. Sci Rep. 2022 Jul 8;12(1):11628.
Time-lapse confocal images of ERK1-EGFP for MEK1-WT or MEK1-mutations after EGF stimulation.
Paper info
Kubota Y, et. al. (2022) Nat Commun. 2022 Jul 13;13(1):4063. / Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M. Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer. Nat Commun. 2022 Jul 13;13(1):4063.
Time-series images of temprature dynamics in HeLa cells.
Paper info
Lu K, et. al. (2022) Nano Lett, 22(14):5698-5707. / Lu K, Wazawa T, Sakamoto J, Vu CQ, Nakano M, Kamei Y, Nagai T. Intracellular Heat Transfer and Thermal Property Revealed by Kilohertz Temperature Imaging with a Genetically Encoded Nanothermometer. Nano Lett. 2022 Jul 27;22(14):5698-5707. doi: 10.1021/acs.nanolett.2c00608.
Live imaging of Ca2+ concentration dynamics in BrafV600E melanoma cells.
Paper info
Konishi Y, et. al. (2021) Cancer Res, 2021 Aug 1;81(15):4124-4132. / Konishi Y, Ichise H, Watabe T, Oki C, Tsukiji S, Hamazaki Y, Murakawa Y, Takaori-Kondo A, Terai K, Matsuda M. Intravital Imaging Identifies the VEGF-TXA2 Axis as a Critical Promoter of PGE2 Secretion from Tumor Cells and Immune Evasion. Cancer Res. 2021 Aug 1;81(15):4124-4132.
Time lapse images of the spatiotemporal regulation of ROCK activity in mice live blood vessels.
Paper info
Imanishi A, et. al. (2021) Am J Pathol, Jan;191(1):194-203. / Imanishi A, Ichise H, Fan C, Nakagawa Y, Kuwahara K, Sumiyama K, Matsuda M, Terai K. Visualization of Spatially-Controlled Vasospasm by Sympathetic Nerve-Mediated ROCK Activation. Am J Pathol. 2021 Jan;191(1):194-203.
Bright-field time-lapse images of live murine immune-related cells defined by scRNA-seq.
Paper info
Jin J, et. al. (2023) Proc Natl Acad Sci U S A. 120(1):e2210283120. / Jin J, Ogawa T, Hojo N, Kryukov K, Shimizu K, Ikawa T, Imanishi T, Okazaki T, Shiroguchi K. Robotic data acquisition with deep learning enables cell image-based prediction of transcriptomic phenotypes. Proc Natl Acad Sci U S A. 2023 Jan 3;120(1):e2210283120.
Spectroscopic OR-PAM images of HeLa cells in the planar-culture or dispersed in Matrigel.
Paper info
Hirasawa T, et. al. (2022) Photoacoustics, May 7;26:100364. / Hirasawa T, Tachi K, Miyashita M, Okawa S, Kushibiki T, Ishihara M. Spectroscopic photoacoustic microscopic imaging during single spatial scan using broadband excitation light pulses with wavelength-dependent time delay. Photoacoustics. 2022 May 7;26:100364.
Fluorescence images of developmental stages of D. magna during early embryogenesis.
Paper info
Adhitama N, et. al. (2020) PLoS One, 2020 Oct 9;15(10):e0239893. / Adhitama N, Kato Y, Matsuura T, Watanabe H. Roles of and cross-talk between ecdysteroid and sesquiterpenoid pathways in embryogenesis of branchiopod crustacean Daphnia magna. PLoS One. 2020 Oct 9;15(10):e0239893.
Time courses images of Ca2+ responses in the TRPC inhibitor-expressing HEK293 cells or HeLa cells.
Paper info
Shimauchi T, et. al. (2022) Cells, Jun 27;11(13):2041. / Shimauchi T, Numaga-Tomita T, Kato Y, Morimoto H, Sakata K, Matsukane R, Nishimura A, Nishiyama K, Shibuta A, Horiuchi Y, Kurose H, Kim SG, Urano Y, Ohshima T, Nishida M. A TRPC3/6 Channel Inhibitor Promotes Arteriogenesis after Hind-Limb Ischemia. Cells. 2022 Jun 27;11(13):2041.
Live imaging of ERK activation on a small epithelial cell cluster during cell migration
Paper info
Asakura Y, et. al. (2021) Sci Rep, 2021 Feb 18;11(1):4069. / Asakura Y, Kondo Y, Aoki K, Naoki H. Hierarchical modeling of mechano-chemical dynamics of epithelial sheets across cells and tissue. Sci Rep. 2021 Feb 18;11(1):4069. doi: 10.1038/s41598-021-83396-6. PMID: 33603023; PMCID: PMC7892579.
Time-lapse STED imaging of mitochondrial dynamics in MitoPB Yellow-labeled cells
Paper info
Wang C, et. al. (2019) Proc Natl Acad Sci U S A., 116(32):15817-15822. / Wang C , Taki M, Sato Y, Tamura Y, Yaginuma H, Okada Y, and Yamaguchi S (2019) A photostable fluorescent marker for the superresolution live imaging of the dynamic structure of the mitochondrial cristae. Proceedings of the National Academy of Sciences of the United States of America, Volume 116, Issue 32, pages 15817-15822.