275-Sasaki-HistonDyn
Author reviewedTime-course fulorescence images of dynamic changes in histone H3K9me3 in living cells.
Project ID
275-Sasaki-HistonDyn
Project SID
275
Title
Time-course fulorescence images of dynamic changes in histone H3K9me3 in living cells.
Description
Histone lysine methylation is an epigenetic mark that can control gene expression. In particular, H3K9me3 contributes to transcriptional repression by regulating chromatin structure. Successful mitotic progression requires correct timing of chromatin structure changes, including epigenetic marks. However, spatiotemporal information on histone modifications in living cells remains limited. In this study, we created an FRET-based probe for live-cell imaging based on the HP1a chromodomain (HP1aCD), which binds to H3K9me3. The probe was incorporated into chromatin and the emission ratio decreased after treatment
with histone methyltransferase inhibitors, indicating that it successfully traced dynamic changes in H3K9me3. Upon entry into mitosis, the probe’s emission ratio transiently increased with a concomitant increase in H3K9me3, then exhibited a stepwise decrease, probably due to loss of HP1aCD binding caused by phosphorylation of H3S10 and demethylation of H3K9me3. This probe will be a useful tool for detecting dynamic changes in chromatin structure associated with HP1a.
Submission Date
2023-03-07
Opened Date
-
Release Date
2023-07-20
Update Date
2023-08-02
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This work was supported in part by a MEXT/JSPS KAKENHI Grant-in-Aid for Scientific
Research (S) (JP19H05640) and a Grant-in-Aid for Scientific Research on Innovative Areas (JP18H05503), and PRESTO, the Japan Science and Technology Agency.
Total
29 files / 1.9 GB
Image datasets
zipped 13 files / 259.3 MB, raw image 16 files / 1.6 GB, total 29 files / 1.9 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Chlorocebus aethiops
(
NCBITaxon:9534
)
Homo sapiens
(
NCBITaxon:9606
)
Mus musculus
(
NCBITaxon:10090
)
Strain
-
Cell
MEFi cells
Cell line
COS-7 cell
(
CLO:0002597
)
HeLa-S3 cell
(
CLO:0003699
)
MeSH
Ontology
Anatomical Entity
-
UBERON
-
Biological Process
mitotic nuclear division
(
GO:0140014
)
Cellular Component
nucleus
(
GO:0005634
)
nucleosome
(
GO:0000786
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
Paper DOI / Paper URL
People
Contact
Kazuki Sasaki
RIKEN
Center for Sustainable Resource Science
-
2-1 Hirosawa, Bioscience Bldg. Rm. S202, Wako, Saitama, 351-0198, Japan
Minoru Yoshida
RIKEN
Center for Sustainable Resource Science
-
2-1 Hirosawa, Bioscience Bldg. Rm. S202, Wako, Saitama, 351-0198, Japan
Imaging dataset contributor
Kazuki Sasaki (RIKEN)
Quantitative dataset contributor
-
Image datasets
13
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-course images of a COS7 cell expressing Hismet-HP1aCD.
Time-series fluorescence images of COS7 cells expressing the CFP-H3.
Time-series fluorescence images of COS7 cells expressing the Hismet-HP1aCD.
Time-series fluorescence images of COS7 cells expressing the K9 reporter.
Time-series fluorescence images of COS7 cells expressing the Hismet-HP1aCD.
Time-series fluorescence images of COS7 cells expressing the Hismet-HP1aCDW41A.
Time-course images of COS7 cells stably expressing Hismet-HP1aCD during mitosis.
Time-course FRET imaging of the nuclei of HeLa S3 cells transiently transfected with Hismet-HP1aFL.
Time-course FRET imaging of the nuclei of HeLa S3 cells transiently transfected with Hismet-HP1aCSD.
Time-course images of HeLa S3 cells expressing Hismet-HP1aCD during mitosis.
Time-course images of MEF cells expressing Hismet-HP1aCD during mitosis.
Chlorocebus aethiops
(
NCBITaxon:9534
)
Time-course images of COS7 cells expressing Hismet-HP1aCD-H3K9R during mitosis.
Chlorocebus aethiops
(
NCBITaxon:9534
)
Time-course images of COS7 cells expressing Hismet-HP1aCD-CDW41A during mitosis.