SSBD:database

Project ID

259-Lu-TemperatureDyn

Title

Time-series images of temprature dynamics in HeLa cells.

Description

Despite improved sensitivity of nanothermometers, direct observation of heat transport inside single cells has remained challenging for the lack of high-speed temperature imaging techniques. Here, we identified insufficient temperature resolution under short signal integration time and slow sensor kinetics as two major bottlenecks. To overcome the limitations, we developed B-gTEMP, a nanothermometer based on the tandem fusion of mNeonGreen and tdTomato fluorescent proteins. We visualized the propagation of heat inside intracellular space by tracking the temporal variation of local temperature at a time resolution of 155 μs and a temperature resolution 0.042 °C. By comparing the fast in situ temperature dynamics with computer-simulated heat diffusion, we estimated the thermal diffusivity of live HeLa cells. The present thermal diffusivity in cells was about 1/5.3 of that of water and much smaller than the values reported for bulk tissues, which may account for observations of heterogeneous intracellular temperature distributions.

Release Date

2023-03-22

Update Date

-

License

CC BY

Version

1.0.0

Method Summary

See details in Lu K, et. al. (2022) Nano Lett, 22(14):5698-5707.

Paper DOI

https://doi.org/10.1021/acs.nanolett.2c00608

Paper URL

https://pubmed.ncbi.nlm.nih.gov/35792763/

Kind

Total

10 files / 5.9 GB

Image datasets

zipped 5 files / 2.1 GB, raw image 5 files / 3.8 GB, total 10 files / 5.9 GB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

-

Cell line

Biological Process

Cellular Component

-

Medical Subject Headings

-

Method involved in biological imaging