274-Aini-MitochondrialDyn
Author reviewedObservation of dynamic mitochondrial distribution during development
and asymmetric division of rice zygotes
Project ID
274-Aini-MitochondrialDyn
Project SID
274
Title
Observation of dynamic mitochondrial distribution during development
and asymmetric division of rice zygotes
Description
Mitochondria are highly dynamic organelles that actively move and change their localization along with actin filaments during the cell cycle. Studies of mitochondrial dynamics and distribution in plant cells have mainly been conducted on somatic cells, and our understanding about these aspects during the formation and development of zygotes remains limited.
In this study, mitochondrial nucleoids of rice egg cells and zygotes were successfully stained by using N-aryl pyrido cyanine 3 (PC3), and their intracellular localization and distribution were demonstrated. Mitochondria in rice egg cells were small and coccoid in shape and were primarily distributed around the nucleus. Upon gamete fusion, the resulting zygotes showed mitochondrial dispersion and accumulation equivalent to those in rice egg cells until 8 h after fusion (HAF). Around 12 HAF, the mitochondria started to disperse throughout the cytoplasm of the zygotes, and this dispersive distribution pattern continued until the zygotes entered the mitotic phase. At early prophase, the mitochondria redistributed from dispersive to densely accumulated around the nucleus, and during the metaphase and anaphase, the mitochondria were depleted from possible mitotic spindle region. Thereafter, during cell plate formation between daughter nuclei, the mitochondria distributed along the phragmoplast, where the new cell wall was formed. Finally, relatively equivalent amounts of mitochondria were detected in the apical and basal cells which were produced through asymmetric division of the zygotes. Further observation by treating the egg cell with latrunculin B revealed that the accumulation of mitochondria around the nuclear periphery in egg cells and early zygotes depended on the actin meshwork converging toward the egg or zygote nucleus.
Submission Date
-
Opened Date
-
Release Date
2023-05-29
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
-
Funding Information
This work was supported, in part, by the MEXT KAKENHI
(Grant-in-Aid for Scientific Research on Innovative Areas, grant
nos. 19H04868 to T.O., 19H05364, 20H05412 to Y.S., 16H06464,
16K21727 to T.H.), and JSPS KAKENHI (Grant-in-Aid for Challeng-
ing Exploratory Research, grant no. 20K21317 to T.O.).
Total
250 files / 239.7 MB
Image datasets
zipped 34 files / 60.3 MB, raw image 216 files / 179.4 MB, total 250 files / 239.7 MB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Strain
-
Cell
Cell line
-
MeSH
Ontology
Anatomical Entity
-
UBERON
zygote stage
(
UBERON:0000106
)
embryo
(
UBERON:0000922
)
Biological Process
mitochondrion distribution
(
GO:0048311
)
Cellular Component
mitochondrion
(
GO:0005739
)
nucleus
(
GO:0005634
)
Molecular Function
-
Medical Subject Headings
Imaging Method
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
time-lapse microscopy
(
FBbi:00000249
)
Paper DOI / Paper URL
People
Contact
Takashi Okamoto
Tokyo Metropolitan University
Department of Biological Sciences
-
Department of Biological Sciences, Tokyo Metropolitan University, Minami-osawa, Hachioji, Tokyo, 192-0397, Japan
Imaging dataset contributor
Hanifah Aini (Tokyo Metropolitan University)
Quantitative dataset contributor
-
Image datasets
34
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 12 h after fusion (HAF) by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 16 h after fusion (HAF) by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 19 h after fusion (HAF) by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 19 h after fusion (HAF) by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 1 h after fusion (HAF) by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 4 h after fusion (HAF) by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 8 h after fusion (HAF) by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice egg cell by PC3 staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 12 h after fusion (HAF) by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 19 h after fusion (HAF) by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 1 h after fusion (HAF) by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 20.5 h after fusion (HAF) by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 20 h after fusion (HAF) by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 4 h after fusion (HAF) by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice zygotes at 8 h after fusion (HAF) by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the development of rice egg cell by MitoTracker Red staining.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the anaphase of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the early telophase of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the early prophase of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during late telophase of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the metaphase of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the mid-telophase of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the prophase of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the two-celled embryo (interphase) of the rice zogotes staining by PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the anaphase of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the early prophase of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the early telophase of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during late telophase of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the metaphase of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the mid-telophase of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the prophase of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Fluorescence images of mitochondrial distribution dynamics during the two-celled embryo (interphase) of the rice zogotes staining by MitoTrackerRed.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
The mitochondrial distribution and movement in rice egg cells with Latrunculin B treatment stained with PC3.
Oryza sativa temperate japonica subgroup
(
NCBITaxon:1736657
)
Time-lapse images of mitochondrial distribution and movement in rice egg cells without Latrunculin B treatment stained with PC3.