295-Katoh-CiliumDyn
Author reviewedImages of immotile cilia dynamics in wild-type mouse embryo or iv/iv mutant embryo
Project ID
295-Katoh-CiliumDyn
Title
Images of immotile cilia dynamics in wild-type mouse embryo or iv/iv mutant embryo
Description
-
Release Date
2023-07-20
Update Date
-
License
Version
1.0.0
Method Summary
See details in Katoh TA, et. al. Science. 2023 Jan 6;379(6627):66-71.
Paper DOI
Kind
Image data
Total
41 files / 32.6 GB
Image datasets
zipped 18 files / 13.5 GB, raw image 23 files / 19.1 GB, total 41 files / 32.6 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
-
Cell line
-
Biological Process
determination of left/right symmetry
(
GO:0007368
)
detection of nodal flow
(
GO:0003127
)
calcium-mediated signaling
(
GO:0019722
)
Cellular Component
non-motile cilium
(
GO:0097730
)
Medical Subject Headings
-
Method involved in biological imaging
highly inclined and laminated optical sheet (HILO) microscopy
Spinning disk confocal microscopy with deconvolution
Spinning disk confocal microscope with custom-built optical pathway
Whole-Cell FRAP
Spinning disk confocal microscopy
time lapse microscopy
(
FBbi:00000249
)
Calcium imaging
Optical-tweezers
STED
(
FBbi:00000334
)
AiryScan
immunostaining
Image datasets
18
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Takanobu A. Katoh
The University of Tokyo
Graduate School of Medicine
Cell Biology
7-3-1, Hongo, Bunkyo-ku, Tokyo, 113-8654, JAPAN
Toshihiro Omori
Tohoku University
Graduate School of Biomedical Engineering
-
-
Hiroshi Hamada
Tata Instituite for Foundamental Research
National Center for Biological Science
-
GKVK Campus, Bellary Road, Bangalore 560065 INDIA
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
HILO imaging of GCaMP6 expressed in immotile cilia at the node of a mouse embryo.
High-resolution microscopy images of immotile cilia at the node after UV irradiation of a wild-type mouse embryo at the two-somite stage.
High-resolution microscopy images of immotile cilia at the node before UV irradiation of a wild-type mouse embryo at the two-somite stage.
Live imaging of nodal flow in a wild-type embryo at the two-somite stage after UV irradiation for 10s.
Live imaging of nodal flow in a wild-type embryo at the two-somite stage after UV irradiation for 20s.
Live imaging of nodal flow in a wild-type embryo at the two-somite stage after UV irradiation for 30s.
Live imaging of nodal flow in a wild-type embryo at the two-somite stage after UV irradiation for 40s.
Live imaging of nodal flow in a wild-type embryo at the two-somite stage after UV irradiation for 50s.
High-resolution microscopy images of nodal flow in a wild-type embryo at the two-somite stage before UV irradiation.
Three-dimension images of immotile cilia in a iv/iv mutant embryo just before stimulation.
Three-dimension images of immotile cilia in a iv/iv mutant embryo just after stimulation.
Time-lapse images of immotile cilia in a iv/iv mutant embryo after stimulation by whole-cell FRAP.
Time-lapse images of immotile cilia in a iv/iv mutant embryo after stimulation by whole-cell FRAP.
Time-lapse images of Ca2+ signalling before stimulation of the immotile cilium of a crown cell
in an iv/iv embryo.
Time-lapse images of Ca2+ signalling under stimulation of the immotile cilium of a crown cell
in an iv/iv embryo.
3D-STED microsocopy imaging of Pkd2 distribution in immotile cilia obtained by STED microscopy.
Time course Ca2+ imaging during dorsal and ventral bending of a cilium.
Superresolution image of acetylated tubulin and mNeonGreen in an embryo harboring the NDE4-hsp-5HT6-mNeonGreen-2A-tdKatushka2 transgene.