SSBD:database

Project ID

273-Kaise-NeuralStemCell

Title

Bioluminescent time-lapse imaging of Ascl1-Luciferse reporter mice's brain slices after iPaD virus injection

Description

Dentate gyrus of 11-month-old luciferase2 - Ascl1 reporter transgenic mice was injected by inducing Plagl2 and anti Dyrk1a (iPaD) - mCherryNLS lentivrus. iPaD - mCherryNLS expressed mainly at adult neural stem cells (NSCs) of dentate gyrus enhancing the proliferation of NSCs where Ascl1 supposed to express in an oscillatory manner. 4 week after iPaD lentivirus injection, brain slices containg dentate gyrus were exracted and placed in a gel supplemented by culture medium. Time-lapse imaging of the bioluminescent signal derived from Luciferse2 - Ascl1 reporter and the mCherry fluorescent signal derived from lentivirus infected cells were detected by Olympus IX-81 with a CCD camera (iKon-M DU934P-BV, Andor) and a 40x objective lens.

Release Date

2024-11-25

Update Date

-

License

CC BY

Version

1.0.0

Method Summary

See details in Kaise T, et. al. Genes Dev. 2022 Jan 1;36(1-2):23-37

Paper DOI

https://doi.org/10.1101/gad.349000.121

Paper URL

https://pubmed.ncbi.nlm.nih.gov/34916302/

Kind

Total

4 files / 291.9 MB

Image datasets

zipped 2 files / 49.9 MB, raw image 2 files / 242.0 MB, total 4 files / 291.9 MB

Quantitative datasets

zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes

Organism

Strain

-

Cell line

-

Biological Process

Cellular Component

-

Medical Subject Headings

-

Method involved in biological imaging