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272-Yoshida-EGFRSignalingDyn
Author reviewed
Time-lapse or z-stack images of EGFR signaling dynamics in epithelial homeostasis of Drosophila embyos.
SSBD:OMERO.gallery
SSBD:OMERO
SSBD:downloads
Project ID
272-Yoshida-EGFRSignalingDyn
Title
Time-lapse or z-stack images of EGFR signaling dynamics in epithelial homeostasis of Drosophila embyos.
Description
-
Release Date
2023-08-29
Update Date
-
License
CC BY
Version
1.0.0
Method Summary
See details in Yoshida K, et. al. Development. 2023 Mar 1;150(5):dev201231.
Paper DOI
https://doi.org/10.1242/dev.201231
Paper URL
https://pubmed.ncbi.nlm.nih.gov/36897356/
Kind
Image data
Total
2417 files / 354.8 GB
Image datasets
zipped 28 files / 44.6 GB, raw image 1152 files / 73.6 GB, total 1180 files / 118.3 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Drosophila melanogaster
(
NCBITaxon:7227
)
Strain
-
Cell line
-
Biological Process
epithelial cell morphogenesis (
GO:0003382
)
tissue homeostasis (
GO:0001894
)
epithelial structure maintenance (
GO:0010669
)
execution phase of apoptosis (
GO:0097194
)
wound healing (
GO:0042060
)
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
time lapse microscopy (
FBbi:00000249
)
confocal microscopy (
FBbi:00000251
)
laser scanning confocal microscope
Image datasets
28
Quantitative datasets
0
Paper DOI / Paper URL
PMID: 36897356
,
doi: 10.1242/dev.201231
OMERO.gallery
https://ssbd.riken.jp/omero/gallery/show_project/1101/
OMERO
https://ssbd.riken.jp/omero/webclient/?show=project-1101
downloads
https://ssbd.riken.jp/data/272-Yoshida-EGFRSignalingDyn/
People
Contact
Shigeo Hayashi
RIKEN
Center for Biosystems Dynamics Research
Laboratory for Morphogenetic Signalin
2-2-3, Minatojima-minamimachi, Chuo-ku, Kobe 650-0047 Japan
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Fig1BC_dextran_injection_ERK
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of rhodamin-labelled dextran in the perivitelline space and ERK-FRET activity in a stage 11 Drosophila embryo.
Fig1D-F_dextran_injection_ERK
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of rhodamin-labelled dextran in the perivitelline space and ERK-FRET activity in a stage 11 Drosophila embryo.
Fig2A_Par6-GFP_control
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse imaging of ventral views of control embryos carrying the junction marker Par6-GFP.
Fig2B_Par6-GFP_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse imaging of ventral views of EGFR-mutant embryos carrying the junction marker Par6-GFP.
Fig2D_Par6-GFP_control
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of lateral views of control embryos carrying the junction marker Par6-GFP.
Fig2E_Par6-GFP_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of lateral views of EGFR-mutant embryos carrying the junction marker Par6-GFP.
Fig3A_DAPI_cDcp1_Dlg_control
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of a control stage12 embryo.
Fig3B_DAPI_cDcp1_Dlg_Df3L
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of a Df(3L)H99 mutant stage12 embryo.
Fig3C_DAPI_cDcp1_Dlg_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of a EGFR mutant stage12 embryo.
Fig3D_DAPI_cDcp1_Dlg_egfr_H99
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of an EGFR/Df(3L)H99 double mutant stage12 embryo.
Fig3E_DAPI_cDcp1_Dlg_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of a EGFR mutant stage10 embryo.
Fig3F_DAPI_cDcp1_Dlg_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of a EGFR mutant stage11 embryo.
Fig3G_Par5-GFP_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of the head region of the EGFR mutant showing an extensive collapse in the ocular segment of the head.
Fig4B_cDcp1_DAP1_Dlg_control
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of a control stage 11 embryo.
Fig4C_cDcp1_DAP1_Dlg_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of a EGFR mutant stage 11 embryo.
Fig4D_cDcp1_DAP1_Dlg_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of an enlarged head parts of EGFR mutant stage 11 embryo.
Fig4E_cDcp1_DAP1_Dlg_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of active caspase staining (cDcp1) of an enlarged thorax parts of EGFR mutant stage 11 embryo.
Fig4F_Par6-GFP_egfr_head
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of the ventral head showing apical cell extrusion in the invaginating salivary gland primordia.
Fig4G_Par6-GFP_egfr_thorax
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of the ventral thorax showing apical cell extrusion in the invaginating salivary gland primordia.
Fig5E_Actin-Gal4_UAS-Apoliner
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of cultured tissue fragments showing apoliner activity in a control tissue fragment.
Fig5F_egfr_Par6-GFP
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of an EGFR mutant tissue fragment undergoing rapid disintegration.
Fig5G_egfr_Par6-GFP
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of the early phase of EGFR mutant tissue culture showing frequent apical cell extrusion.
Fig6A_HS-GFP_his-RFP_before
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of epithelial tissue before infrared laser illumination.
Fig6B_HS-GFP_his-RFP_after
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of tissue wound occur after infrared laser illumination.
Fig6C_dpERK_FRET_cytoplasm
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Z-stack image of ERK activation in the vicinity of the wound region.
Fig6D_ERK_FRET_Sqh-mcherry
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of ERK activity and myosin activity.
Fig6E_Par6-GFP_his-RFP_control
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of laser wound damage in a control embryo.
Fig6F_Par6-GFP_his-RFP_egfr
Drosophila melanogaster
(
NCBITaxon:7227
)
Image data
omero.gallery
omero
Time-lapse images of laser wound damage in a EGFR mutant.