Unified Projects

276 items found.
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2026-01-21
146
raw_image 689 files / 77.0 GB
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v3
Title
Visual quantification of prostaglandin E2 discharge from a single cell
Paper info
Watabe T, Yamahira S, Matsuda M, Terai K. Visual quantification of prostaglandin E2 discharge from a single cell. Cell Struct Funct . 2023 Dec 7;48(2):241-249.

2026-01-21
17
raw_image 17 files / 17.6 GB
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v3
Title
Dynamics of pollen tube in Arabidopsis thaliana
Paper info
Mizuta Y, Sakakibara D, Nagahara S, Kaneshiro I, Nagae TT, Kurihara D, Higashiyama T. Deep imaging reveals dynamics and signaling in one-to-one pollen tube guidance. EMBO Rep. 2024 Jun;25(6):2529-2549.

Title
Dynamics of radial axis formation in Arabidopsis thaliana zygote
Paper info
Tanaka S, Matsushita Y, Hanaki Y, Higaki T, Kamamoto N, Matsushita K, Higashiyama T, Fujimoto K, Ueda M. HD-ZIP IV genes are essential for embryo initial cell polarization and the radial axis formation in Arabidopsis. Curr Biol. 2024 Oct 21;34(20):4639-4649.e4.

2025-12-03
17
raw_image 24140 files / 8.3 GB
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v3
Title
Time-lapse images of collective cell migration regulated by extracellular matrix
Paper info
Hagiwara M, Maruyama H, Akiyama M, Koh I, Arai F. Weakening of resistance force by cell-ECM interactions regulate cell migration directionality and pattern formation. Commun Biol . 2021 Jun 28;4(1):808.

2025-12-03
468
raw_image 32990 files / 624.7 GB
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v3
Title
Live chromosome identifying and tracking in mouse oocytes
Paper info
Takenouchi O, Sakakibara Y, Kitajima TS. Live chromosome identifying and tracking reveals size-based spatial pathway of meiotic errors in oocytes. Science. 2024 Jul 19;385(6706):eadn5529.

2025-11-28
23
raw_image 16747 files / 353.0 GB
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Title
The folding dynamics of Drosophila wing during pupal development
Paper info
A. Tsuboi, K. Fujimoto, T. Kondo. Spatiotemporal remodeling of extracellular matrix orients epithelial sheet folding. Sci Adv. 2023 Sep;9(35):eadh2154

2025-11-28
10
raw_image 1 files / 3.5 GB
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v3
Title
Immunofluorescence images of nerve fiber and IL-31 receptors in the mouse ear skin
Paper info
Takahashi S, Ochiai S, Jin J, Takahashi N, Toshima S, Ishigame H, Kabashima K, Kubo M, Nakayama M, Shiroguchi K, Okada T. Sensory neuronal STAT3 is critical for IL-31 receptor expression and inflammatory itch. Cell Rep. 2023 Dec 26;42(12):113433.

2025-11-28
11
raw_image 62 files / 397.1 MB
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v3
Title
3D-time-lapse images of vacuolar sorting of BOR1
Paper info
Yoshinari A, Shimizu Y, Hosokawa T, Nakano A, Uemura T, Takano J. Rapid Vacuolar Sorting of the Borate Transporter BOR1 Requires the Adaptor Protein Complex AP-4 in Arabidopsis. Plant Cell Physiol. 2024 Dec 6;65(11):1801-1811.

2025-11-28
20
raw_image 24 files / 48.9 GB
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v3
Title
BMP4 regulates asymmetric Pkd2 distribution in mouse nodal immotile cilia and ciliary mechanosensing required for left–right determination
Paper info
Katoh TA, Lange T, Nakajima Y, Yashiro K, Okada Y, Hamada H. BMP4 regulates asymmetric Pkd2 distribution in mouse nodal immotile cilia and ciliary mechanosensing required for left-right determination. Dev Dyn. 2024 Jul 10.

2025-11-28
20
raw_image 50 files / 348.6 GB
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v3
Title
Images of brain and xenograft obtained by descSPIM, the desktop- equipped SPIM
Paper info
Otomo K, Omura T, Nozawa Y, Edwards SJ, Sato Y, Saito Y, Yagishita S, Uchida H, Watakabe Y, Naitou K, Yanai R, Sahara N, Takagi S, Katayama R, Iwata Y, Shiokawa T, Hayakawa Y, Otsuka K, Watanabe-Takano H, Haneda Y, Fukuhara S, Fujiwara M, Nii T, Meno C, Takeshita N, Yashiro K, Rosales Rocabado JM, Kaku M, Yamada T, Oishi Y, Koike H, Cheng Y, Sekine K, Koga JI, Sugiyama K, Kimura K, Karube F, Kim H, Manabe I, Nemoto T, Tainaka K, Hamada A, Brismar H, Susaki EA. descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques. Nat Commun. 2024 Jun 12;15(1):4941.

2025-11-28
181
raw_image 20061 files / 1.2 TB
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v3
Title
Dynamics of chromosomes and artificial kinetochore beads in mouse oocytes and embryos
Paper info
Asai K, Zhou Y, Takenouchi O, Kitajima TS. Artificial kinetochore beads establish a biorientation-like state in the spindle. Scince . 2024 Sep 20;385(6715):1366-1375.

2025-11-28
8
raw_image 8 files / 506.3 MB
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Title
EGFR dynamics on CHO–K1 cells with depletion and supplementation of cholesterol
Paper info
Takayama M, Maeda S, Watanabe D, Takebayashi K, Hiroshima M, Ueda M. Cholesterol suppresses spontaneous activation of EGFR-mediated signal transduction. Biochem Biophys Res Commun . 2024 Apr 16:704:149673.

2025-11-28
6
raw_image 6 files / 619.2 MB
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v3
Title
The shape of vacuole and F-actin in tobacco BY-2 cells treated with neopetromin, a newly found peptide
Paper info
Hitora Y, El-Desoky AH, Sadahiro Y, Sejiyama A, Kinoshita A, Ise Y, Angkouw ED, Mangindaan REP, Higaki T, Tsukamoto S. Neopetromin, a Cyclic Tripeptide with a C-N Cross-Link, from the Marine Sponge Neopetrosia sp., That Causes Vacuole Fragmentation in Tobacco BY-2 Cells. J Nat Prod. 2024 Apr 26;87(4):1197-1202.

2025-11-28
7
raw_image 56986 files / 22.5 GB
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v2
Title
Aquaporin overexpression promotes endothelial-to-hematopoietic transition.
Paper info
Sato Y, et. al. (2023) Development. Jun 1;150(11):dev201275. / Sato Y, Shigematsu M, Shibata-Kanno M, Maejima S, Tamura C, Sakamoto H. Aquaporin regulates cell rounding through vacuole formation during endothelial-to-hematopoietic transition. Development. 2023 Jun 1;150(11):dev201275.

2025-11-26
24
raw_image 132 files / 8.4 GB
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v3
Title
Time-lapse images of intracellular structure in elongating Arabidopsis zygotes
Paper info
Hiromoto Y, Minamino N, Kikuchi S, Kimata Y, Matsumoto H, Nakagawa S, Ueda M, Higaki T. Comprehensive and quantitative analysis of intracellular structure polarization at the apical-basal axis in elongating Arabidopsis zygotes. Sci Rep. 2023 Dec 18;13(1):22879.

2025-11-26
31
raw_image 265 files / 153.6 GB
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v3
Title
Super resolution cellular images using fluorescent-tags of StayGold variants
Paper info
Ando R, Shimozono S, Ago H, Takagi M, Sugiyama M, Kurokawa H, Hirano M, Niino Y, Ueno G, Ishidate F, Fujiwara T, Okada Y, Yamamoto M, Miyawaki A. StayGold variants for molecular fusion and membrane-targeting applications. Nat Methods. 2024 Apr;21(4):648-656.

2025-11-26
85
raw_image 85 files / 7.0 GB
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v3
Title
Climbing fiber inputs to Purkinje cells in wild type mice and PTPdelta knockout mice
Paper info
Okuno Y, Sakoori K, Matsuyama K, Yamasaki M, Watanabe M, Hashimoto K, Watanabe T, Kano M. PTPdelta is a presynaptic organizer for the formation and maintenance of climbing fiber to Purkinje cell synapses in the developing cerebellum. Front Mol Neurosci. 2023 Jun 22:16:1206245.

2025-11-26
9
raw_image 44 files / 2.0 GB
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Title
SARS-CoV-2 infection to the co-culture of bronchial organoids and vascular beds in a microfluidic device
Paper info
Fujimoto K, Kameda Y, Nagano Y, Deguchi S, Yamamoto T, Krol RP, Gee P, Matsumura Y, Okamoto T, Nagao M, Takayama K, Yokokawa R. SARS-CoV-2-induced disruption of a vascular bed in a microphysiological system caused by type-I interferon from bronchial organoids. Lab Chip . 2024 Aug 6;24(16):3863-3879.

2025-11-26
11
raw_image 18 files / 263.0 GB
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v3
Title
Z-series and time-lapse images showing phyllotaxis-like periodic and symmetric tentacle arrangements
Paper info
Sarper SE, Kitazawa MS, Nakanishi T, Fujimoto K. Size-correlated polymorphisms in phyllotaxis-like periodic and symmetric tentacle arrangements in hydrozoan Coryne uchidai. Front Cell Dev Biol. 2023 Nov 22:11:1284904.

2025-11-26
7
raw_image 5124 files / 633.7 GB
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v3
Title
Ultra-large FOV imaging by AMATERAS-2
Paper info
Ichimura T, Kakizuka T, Taniguchi Y, Ejima S, Sato Y, Itano K, Seiriki K, Hashimoto H, Sugawara K, Itoga H, Onami S, Nagai T. Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo. Elife. 2025 Feb 3;13:RP93633

2025-04-21
22
raw_image 22 files / 1.1 GB
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v3
Title
Shoot gravitropism of wild type and myosin xif xik mutant Arabidopsis
Paper info
Tsugawa S, Miyake Y, Okamoto K, Toyota M, Yagi H, Terao Morita M, Hara-Nishimura I, Demura T, Ueda H. Shoot gravitropism and organ straightening cooperate to arrive at a mechanically favorable shape in Arabidopsis. Sci Rep. 2023 Jul 17;13(1):11165.

2025-04-21
8
raw_image 8 files / 1.9 GB
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Title
3D images of bilaminar organoid generated from co-culture of human pluripotent stem cells (hPSC) and hPSC-derived hypoblast-like cells
Paper info
Okubo T, Rivron N, Kabata M, Masaki H, Kishimoto K, Semi K, Nakajima-Koyama M, Kunitomi H, Kaswandy B, Sato H, Nakauchi H, Woltjen K, Saitou M, Sasaki E, Yamamoto T, Takashima Y. Hypoblast from human pluripotent stem cells regulates epiblast development. Nature. 2024 Feb;626(7998):357-366.

2025-04-10
125
raw_image 2231 files / 20.9 GB
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v3
Title
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Paper info
Katsuta H, Okuda S, Nagayama K, Machiyama H, Kidoaki S, Kato M, Sokabe M, Miyata T, Hirata H. Actin crosslinking by alpha-actinin averts viscous dissipation of myosin force transmission in stress fibers. iScience. 2023 Feb1;26(3):106090.

Title
Stimulated Raman images of deuterated methionine incorporated into adult and larval Drosophila
Paper info
Spratt SJ, Mizuguchi T, Akaboshi H, Kosakamoto H, Okada R, Obata F, Ozeki Y. Imaging the uptake of deuterated methionine in Drosophila with stimulated Raman scattering. Front Chem. 2023 Mar 29;11:1141920.

2025-04-10
22
raw_image 22 files / 26.6 GB
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Title
Time-lapse images of MDS-derived AML cell lines treated with decitabine
Paper info
Yabushita T, Chinen T, Nishiyama A, Asada S, Shimura R, Isobe T, Yamamoto K, Sato N, Enomoto Y, Tanaka Y, Fukuyama T, Satoh H, Kato K, Saitoh K, Ishikawa T, Soga T, Nannya Y, Fukagawa T, Nakanishi M, Kitagawa D, Kitamura T, Goyama S. Mitotic perturbation is a key mechanism of action of decitabine in myeloid tumor treatment. Cell Rep. 2023 Sep 26;42(9):113098.

2025-04-10
39
raw_image 39 files / 4.7 GB
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Title
Calcium imaging of ASEL gustatory neuron of C. elagans in response to glucose after conditioning
Paper info
Tomioka M, Umemura Y, Ueoka Y, Chin R, Katae K, Uchiyama C, Ike Y, Iino Y. Antagonistic regulation of salt and sugar chemotaxis plasticity by a single chemosensory neuron in Caenorhabditis elegans. PLoS Genet. 2023 Sep 5;19(9):e1010637. PLoS Genet. 2023 Sep 5;19(9):e1010637.

2025-03-28
174
raw_image 4627959 files / 2.2 TB
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v2
Title
Live-cell imaging and BDML file for quantitative information about nucleus dynamics in mouse embryo development.
Paper info
Mashiko D, et. al. Sci Rep. 2022 Jun 7;12(1):9411. / Mashiko D, Ikeda Z, Tokoro M, Hatano Y, Yao T, Kobayashi TJ, Fukunaga N, Asada Y, Yamagata K. Asynchronous division at 4-8-cell stage of preimplantation embryos affects live birth through ICM/TE differentiation. Sci Rep. 2022 Jun 7;12(1):9411.

2025-02-17
184
raw_image 19820 files / 9.8 GB
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v2
Title
Live-cell imaging of G-protein-coupled receptors (GPCRs) downstream signaling dynamics in HeLa cells.
Paper info
Tany R, et. al. Biochem J. 2022 Apr 29;479(8):883-900. / Tany R, Goto Y, Kondo Y, Aoki K. Quantitative live-cell imaging of GPCR downstream signaling dynamics. Biochem J. 2022 Apr 29;479(8):883-900.

2025-02-13
17
raw_image 1964 files / 14.5 GB
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v3
Title
Spatio-temporal activation of ERK during branching morphogenesis in lung
Paper info
Hirashima T and Matsuda M. ERK-mediated curvature feedback regulates branching morphogenesis in lung epithelial tissue. Curr Biol. 2024 Feb 26;34(4):683-696.e6.

2025-01-31
32
raw_image 572 files / 462.1 MB
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v3
Title
TEM images of alpha-synuclein released in serum
Paper info
Okuzumi A, Hatano T, Matsumoto G, Nojiri S, Ueno SI, Imamichi-Tatano Y, Kimura H, Kakuta S, Kondo A, Fukuhara T, Li Y, Funayama M, Saiki S, Taniguchi D, Tsunemi T, McIntyre D, Gerardy JJ, Mittelbronn M, Kruger R, Uchiyama Y, Nukina N, Hattori N. Propagative alpha-synuclein seeds as serum biomarkers for synucleinopathies. Nat Med. 2023 Jun;29(6):1448-1455.

2025-01-31
33
raw_image 153 files / 463.9 MB
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v3
Title
Ammonium chemotaxis of Chlamydomonas reinhardtii
Paper info
Nelson G, Strain A, Isu A, Rahnama A, Wakabayashi KI, Melvin AT, Kato N. Cells collectively migrate during ammonium chemotaxis in Chlamydomonas reinhardtii. Sci Rep. 2023 Jul 4;13(1):10781

2025-01-31
3
raw_image 15 files / 151.7 KB
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v3
Title
Multiple deuteration of triphenylphosphine and live-cell Raman imaging of deuterium-incorporated Mito-Q
Paper info
Moriyama S, Mae M, Shibata D, Yamakoshi H, Kajimoto S, Nakabayashi T, Ishimoto T, Mogi K, Sajiki H, Akai S, Sawama Y. Multiple deuteration of triphenylphosphine and live-cell Raman imaging of deuterium-incorporated Mito-Q. Chem Commun (Camb). 2023 Oct 10;59(81):12100-12103.

2025-01-31
2
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v3
Title
Raman spectra data of cell spheroids on a multi-well plate treated with various concentrations of reagents
Paper info
Liao HX, Bando K, Li M, Fujita K. Multifocal Raman Spectrophotometer for Examining Drug-Induced and Chemical-Induced Cellular Changes in 3D Cell Spheroids. Anal Chem. 2023 Oct 3;95(39):14616-14623.

2025-01-31
1
raw_image 465 files / 930.2 MB
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v3
Title
Beta-tubulin dynamics during cell cycles of red algae
Paper info
Ichinose TM, Iwane AH. Long-term live cell cycle imaging of single Cyanidioschyzon merolae cells. Protoplasma. 2021 May;258(3):651-660.

2025-01-31
6
raw_image 6 files / 1.0 MB
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Title
Raman images showing thia-Michael reaction in HeLa cells
Paper info
Yamakoshi H, Shibata D, Bando K, Kajimoto S, Kohyama A, Egoshi S, Dodo K, Iwabuchi Y, Sodeoka M, Fujita K, Nakabayashi T. Ratiometric analysis of reversible thia-Michael reactions using nitrile-tagged molecules by Raman microscopy. Chem Commun (Camb). 2023 Dec 7;59(98):14563-14566.

2025-01-31
14
raw_image 14 files / 8.2 GB
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Title
Mechanical regulation of DNA underwinding in the nucleus
Paper info
Fukute J, Maki K, Adachi T. The nucleolar shell provides anchoring sites for DNA untwisting. Commun Biol. 2024 Jan 23;7(1):83.

2024-12-24
6
raw_image 12 files / 1.9 TB
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v3
Title
Time-lapse images showing the role of mitochondrial TERT during apoptosis
Paper info
Ebata H, Shima T, Iizuka R, Uemura S. Accumulation of TERT in mitochondria exerts two opposing effects on apoptosis. FEBS Open Bio. 2023, Sep;13(9) :1667-1682.

2024-12-23
3
raw_image 1623 files / 1.2 GB
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v3
Title
Fluorescent labeling of cell-cell interaction using sGRAPHIC
Paper info
Minegishi M, Kuchimaru T, Nishikawa K, Isagawa T, Iwano S, Iida K, Hara H, Miura S, Sato M, Watanabe S, Shiomi A, Mabuchi Y, Hamana H, Kishi H, Sato T, Sawaki D, Sato S, Hanazono Y, Suzuki A, Kohro T, Kadonosono T, Shimogori T, Miyawaki A, Takeda N, Shintaku H, Kizaka-Kondoh S, Nishimura S Secretory GFP reconstitution labeling of neighboring cells interrogates cell-cell interactions in metastatic niches. Nat Commun. 2023 Dec 5;14(1):8031.

2024-12-23
52
raw_image 4661 files / 50.1 GB
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v2
Title
Aggregates formation of Drosophila melanogaster Argonaute2 N-terminal region
Paper info
Narita H, et. al. BMC Biol. 2023 Apr 19;21(1):78. / Narita H, Shima T, Iizuka R, Uemura S. N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates. BMC Biol. 2023 Apr 19;21(1):78.

2024-12-14
6
raw_image 75 files / 1.6 GB
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v3
Title
KIF5C running on various states of microtubules
Paper info
Shima T, Morikawa M, Kaneshiro J, Kambara T, Kamimura S, Yagi T, Iwamoto H, Uemura S, Shigematsu H, Shirouzu M, Ichimura T, Watanabe TM, Nitta R, Okada Y, Hirokawa N. Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport. J Cell Biol. 2018 Dec 3;217(12):4164-4183.

2024-12-14
20
raw_image 20 files / 70.0 GB
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v3
Title
In vivo imaging of the phagocytic dynamics underlying efficient clearance of adult-born hippocampal granule cells by ramified microglia
Paper info
Kamei R, Okabe S. In vivo imaging of the phagocytic dynamics underlying efficient clearance of adult-born hippocampal granule cells by ramified microglia. Glia. 2023 Aug;71(8):2005-2023.

2024-12-14
89
raw_image 93 files / 12.4 GB
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v3
Title
3D medaka brain visualized clonally related units in the pallium
Paper info
Isoe Y, Nakamura R, Nonaka S, Kamei Y, Okuyama T, Yamamoto N, Takeuchi H, Takeda H. Epigenetically distinct synaptic architecture in clonal compartments in the teleostean dorsal pallium. Elife. 2023 Jul 25;12:e85093. doi: 10.7554/eLife.85093.

2024-12-14
10
raw_image 30 files / 665.3 MB
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v3
Title
Influence of persistent SARS-CoV-2 infection on a three- dimensional cardiac model
Paper info
Murata K. et al. Predicted risk of heart failure pandemic due to persistent SARS-CoV-2 infection using a three-dimensional cardiac model. iScience. 2023 Dec 22;27(1):108641.

2024-12-14
3
raw_image 4 files / 3.4 GB
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v3
Title
Changes of tension in smooth muscle cell after treatment with water, calyculin A, or Y27632
Paper info
Wang J, Maeda E, Tsujimura Y, Abe T, Kiyonari H, Kitaguchi T, Yokota H, Matsumoto T. In situ FRET measurement of cellular tension using conventional confocal laser microscopy in newly established reporter mice expressing actinin tension sensor. Sci Rep. 2023 Dec 20;13(1):22729.

2024-12-14
22
raw_image 62 files / 128.5 MB
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v2
Title
Images of OCT and FAG after transplantation of monkey iPSC-retina
Paper info
Uyama H, et. al. Stem Cell Reports. 2022 Nov 8;17(11):2392-2408. / Uyama H, Tu HY, Sugita S, Yamasaki S, Kurimoto Y, Matsuyama T, Shiina T, Watanabe T, Takahashi M, Mandai M. Competency of iPSC-derived retinas in MHC-mismatched transplantation in non-human primates. Stem Cell Reports. 2022 Nov 8;17(11):2392-2408.

2024-12-14
16
raw_image 21 files / 61.1 MB
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v2
Title
Immunofluorescence images or transmission electron images of SARS-CoV-2 infected human induced salivary glands (hiSGs).
Paper info
Tanaka J, et. al. (2022) Nat Cell Biol. 2022 Nov;24(11):1595-1605. / Tanaka J, Senpuku H, Ogawa M, Yasuhara R, Ohnuma S, Takamatsu K, Watanabe T, Mabuchi Y, Nakamura S, Ishida S, Sadaoka T, Takaki T, Shirota T, Shimane T, Inoue T, Sakai T, Mori M, Tsuji T, Saito I, Mishima K. Human induced pluripotent stem cell-derived salivary gland organoids model SARS-CoV-2 infection and replication. Nat Cell Biol. 2022 Nov;24(11):1595-1605.

2024-12-14
24
raw_image 24 files / 13.4 GB
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v2
Title
Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species
Paper info
Kimata Y, et. al. Life Sci Alliance. 2023 Feb 27;6(5):e202201657. / Kimata Y, Yamada M, Murata T, Kuwata K, Sato A, Suzuki T, Kurihara D, Hasebe M, Higashiyama T, Ueda M.. Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species. Life Sci Alliance. 2023 Feb 27;6(5):e202201657.

2024-12-14
14
raw_image 46 files / 1.1 GB
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v2
Title
Endosome maturation promoted by interorganelle association between mitochondria and endosomes
Paper info
Satoh AO, et. al. Cell Rep. 2023 Mar 28;42(3):112229. / Satoh AO, Fujioka Y, Kashiwagi S, Yoshida A, Fujioka M, Sasajima H, Nanbo A, Amano M, Ohba Y. Interaction between PI3K and the VDAC2 channel tethers Ras-PI3K-positive endosomes to mitochondria and promotes endosome maturation. Cell Rep. 2023 Mar 28;42(3):112229.

2024-12-14
8
raw_image 31 files / 73.7 MB
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v2
Title
Human adipose mesenchymal stem cells
Paper info
Yamamoto T, et. al. Stem Cells Transl Med. 2023 Mar 17;12(3):169-182. / Yamamoto T, Arita M, Tamura T, Saito M, Katayama H, Kuroda H, Suzuki T, Kawamata S. A Novel Approach for Determining the Critical Quality Attributes of Mesenchymal Stem Cells by Specifying Cell Population With Replication Potential. Stem Cells Transl Med. 2023 Mar 17;12(3):169-182.

2024-12-14
4
raw_image 4 files / 15.7 GB
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v2
Title
Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes
Paper info
Kimata Y, et. al. Quant Plant Biol. 2020 Nov 30;1:e3. / Kimata Y, Higaki T, Kurihara D, Ando N, Matsumoto H, Higashiyama T, Ueda M. Mitochondrial dynamics and segregation during the asymmetric division of Arabidopsis zygotes. Quant Plant Biol. 2020 Nov 30;1:e3.