282-Ohgushi-StemCell
Author reviewedEmbryonic stem cell differentiation
Project ID
282-Ohgushi-StemCell
Title
Embryonic stem cell differentiation
Description
Chemical blockage of ACTIVIN/NODAL and FGF signals induces human primed ESCs into GATA3-expressing cells that give rise to placental hormone-producing syncytia analogous to syncytiotrophoblasts of the post-implantation stage of the human embryo.
Release Date
2024-11-25
Update Date
-
License
Version
1.0.0
Method Summary
See details in Ohgushi M, et. al. Cell Rep. 2022 Jun 21;39(12):110973
Kind
Image data
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell line
-
Biological Process
morphogenesis of an epithelium
(
GO:0002009
)
stem cell differentiation
(
GO:0048863
)
syncytium formation
(
GO:0006949
)
cell division
(
GO:0051301
)
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
confocal microscopy
(
FBbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
4
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Masatoshi Ohgushi
Kyoto University
Institute for Life and Medical Sciences
Laboratory of Organoids Technology, Center for Human ES Cell Research
-
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Z-stack images of the KhES-1 colonies treated or untreated with AP for 2 days
Live cell image for ESCs (human primed embryonic stem cells) into GATA3-expressing cells
Time-lapse images of syncytium formation from ESCs (human primed embryonic stem cells)
Confocal images for tracking cell division from day 2–5 during AP-induced differentiation