299-Satoh-Endosome
Author reviewedEndosome maturation promoted by interorganelle association between mitochondria and endosomes
Project ID
299-Satoh-Endosome
Title
Endosome maturation promoted by interorganelle association between mitochondria and endosomes
Description
Intracellular organelles of mammalian cells communicate with one another during various cellular processes. The functions and molecular mechanisms of such interorganelle association remain largely unclear, however. The authors identify voltage-dependent anion channel 2 (VDAC2), a mitochondrial outer membrane protein, as a binding partner of phosphoinositide 3-kinase (PI3K), a regulator of clathrin-independent endocytosis downstream of the small GTPase Ras. VDAC2 tethers endosomes positive for the Ras-PI3K complex to mitochondria in response to cell stimulation with epidermal growth factor and promotes clathrin-independent endocytosis, as well as endosome maturation at membrane association sites. With an optogenetics system to induce mitochondrion-endosome association, the authors find that, in addition to its structural role in such association, VDAC2 is functionally implicated in the promotion of endosome maturation.
Release Date
2024-12-14
Update Date
-
License
Version
1.0.0
Method Summary
See details in Satoh AO, et. al. Cell Rep. 2023 Mar 28;42(3):112229.
Kind
Image data
Total
60 files / 1.5 GB
Image datasets
zipped 14 files / 362.3 MB, raw image 46 files / 1.1 GB, total 60 files / 1.5 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell line
A431 cell
(
CLO:0001591
)
Biological Process
organelle assembly
(
GO:0070925
)
endosomal lumen acidification
(
GO:0048388
)
epidermal growth factor receptor signaling pathway
(
GO:0007173
)
Cellular Component
early endosome
(
GO:0005769
)
mitochondrion
(
GO:0005739
)
endosome
(
GO:0005768
)
late endosome
(
GO:0005770
)
Medical Subject Headings
-
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
confocal microscopy
(
Fbbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
Image datasets
14
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Yusuke Ohba
Hokkaido University
Department of Cell Physiology
-
-
Imaging dataset contributor
Aya O. Satoh
(Hokkaido University)
Yoichiro Fujioka
(Hokkaido University)
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of early endosome that did not associate with mitochondria in A431 cells expressing mito-SECFP, VN-H-Ras-WT, PI3K-RBD-VC, and TagRFP-EEA1 after stimulation with EGF
Time-lapse images of A431 cells incubated first with MitoTracker Green and PlasMem Bright Red and then stimulated with EGF
Time-lapse images of A431 cells transfected with expression vectors for CRY2-iRFP-FYVE and TOM20-mCherry-CIB1 exposed to 488-nm light
Time-lapse images of siControl A431 cells transfected with expression vectors for CRY2-MiCy-FYVE and TOM20-iRFPCIB1 incubated with AcidiFluor-Dx exposed to 488-nm light.
Time-lapse images of siVDAC2 A431 cells transfected with expression vectors for CRY2-MiCy-FYVE and TOM20-iRFPCIB1 incubated with AcidiFluor-Dx exposed to 488-nm light.
Time-lapse images of A431 cells transfected with expression vectors for mitochondrially targeted SECFP (mito-SECFP), VN-H-Ras-WT, PI3K-RBD-VC, and TagRFP-EEA1 after stimulation with EGF
Time-lapse images of A431 cells transfected with expression vectors for mito-SECFP, Venus-EEA1, and mCherry-Rab7 after stimulation with EGF
Time-lapse images of A431 cells transfected with expression vectors for mito-SECFP, Venus-EEA1, and mCherry-Rab7 after stimulation with EGF
Time-lapse images of the Ras-PI3K complex in control siRNA-transfected cells stimulated with EGF
Time-lapse images of the Ras-PI3K complex in VDAC2 siRNA-transfected cells stimulated with EGF
Time-lapse images of A431 cells transfected with expression vectors for mitochondrially targeted SECFP (mito-SECFP), VN-H-Ras-WT, PI3K-RBD-VC, and TagRFP-EEA1 after stimulation with EGF
Time-lapse images of A431 cells transfected with expression vectors for mito-SECFP, Venus-EEA1, and mCherry-Rab7 after stimulation with EGF
Time-lapse imaging of endosome acidification dependent on mitochondrion-endosome association in control siRNA-transfected cells
Time-lapse imaging of endosome acidification dependent on mitochondrion-endosome association in VDAC2 siRNA-transfected cells