316-Ando-CellCycle
Author reviewedSharp visualization of cell-cycle progression using Fucci(SA)5 and Fucci(CA)5 fluorescent proteins
Project ID
316-Ando-CellCycle
Title
Sharp visualization of cell-cycle progression using Fucci(SA)5 and Fucci(CA)5 fluorescent proteins
Description
In the present study, we developed practically useful GFP and RFP named h2-3 and AzaleaB5, respectively, from corals, and substituted this GFP/RFP pair for the mVenus/mCherry pair in both Fucci(SA)2 and Fucci(CA)2 probes. The resultant probes: Fucci(SA)5 and Fucci(CA)5, respectively, could monitor cell-cycle progression in HeLa cells.
Release Date
2024-11-25
Update Date
-
License
Version
1.0.0
Method Summary
See details in Ando R, et. al. Cell Struct Funct. 2023 Jul 29;48(2):135-144
Paper DOI
Kind
Image data
Organism
Homo sapiens
(
NCBITaxon:9606
)
Strain
-
Cell line
HeLa/Fucci(SA)5 (clone #16) (RCB 4917)
HeLa/Fucci(CA)5 (clone #16) (RCB 4919)
Biological Process
cell cycle phase transition
(
GO:0044770
)
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
time lapse microscopy
(
FBbi:00000249
)
fluorescence microscopy
(
FBbi:00000246
)
Image datasets
2
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Asako Sakaue-Sawano
RIKEN
Center for Brain Science
Laboratory for Cell Function Dynamics
2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.
Atsushi Miyawaki
RIKEN
Center for Brain Science
Laboratory for Cell Function Dynamics
2-1 Hirosawa, Wako-city, Saitama 351-0198, Japan.
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse image of Fucci(SA)5 fluorescent protein in live HeLa cells
Time-lapse image of Fucci(CA)5 fluorescent protein in live HeLa cells