319-Shou-RESORT
Author reviewedSuper-resolution vibrational imaging based on photoswitchable Raman probe
Project ID
319-Shou-RESORT
Title
Super-resolution vibrational imaging based on photoswitchable Raman probe
Description
The authors first describe a bright photoswitchable Raman probe (DAE620) and validate its signal activation and depletion characteristics when exposed to low-power (microwatt level) continuous-wave laser light. By harnessing the SRS signal depletion of DAE620 through a donut-shaped beam, they demonstrate super-resolution vibrational imaging of mammalian cells with excellent chemical spe- cificity and spatial resolution beyond the optical diffraction limit. These results indicate RESORT microscopy to be an effective tool with high potential for multiplexed super-resolution imaging of live cells.
Release Date
2024-11-25
Update Date
-
License
Version
1.0.0
Method Summary
See details in Shou J, et. al. Sci Adv. 2023 Jun 16;9(24):eade9118.
Paper DOI
Kind
Image data
Organism
Homo sapiens
(
NCBITaxon:9606
)
Drosophila
(
NCBITaxon:7215
)
Strain
-
Cell line
HeLa cell
(
CLO:0003684
)
Biological Process
-
Cellular Component
mitochondrion
(
GO:0005739
)
Medical Subject Headings
-
Method involved in biological imaging
inelastic scattering of photons (Raman scattering)
(
FBbi:00000589
)
reversible saturable optical Raman transitions (RESORT) microscopy
Image datasets
11
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Yasuyuki Ozeki
The University of Tokyo
Department of Electrical Engineering and Information Systems
-
-
Mako Kamiya
The University of Tokyo
Graduate School of Medicine
-
-
Imaging dataset contributor
Jingwen Shou
(The University of Tokyo)
Ayumi Komazawa
(The University of Tokyo)
Yuusaku Wachi
(The University of Tokyo)
Hiroyoshi Fujioka
(The University of Tokyo)
Minoru Kawatani
(The University of Tokyo)
Takaha Mizuguchi
(The University of Tokyo)
Hikaru Akaboshi
(The University of Tokyo)
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Photoswitchable SRS signal of fixed HeLa cells stained with DAE620 observed at the Raman on-resonance wave number
Photoswitchable SRS signal of fixed HeLa cells stained with DAE620 observed at the Raman off-resonance wave number
Normal SRS imaging of fixed HeLa cells stained with DAE620-Mito
RESORT imaging of fixed HeLa cells stained with DAE620-Mito
Normal SRS imaging of live HeLa cells stained with DAE620-Mito
RESORT imaging of live HeLa cells stained with DAE620-Mito
Photoswitchable SRS imaging of fixed HeLa cells stained by DAE620-Mito observed at the Raman on-resonance wave number
Photoswitchable SRS imaging of fixed HeLa cells stained by DAE620-Mito observed at the Raman off-resonance wave number
Photoswitchable SRS spectra of fixed HeLa cells stained with DAE620
Photoswitchable SRS imaging of Drosophila larval fat bodies stained with DAE620 observed at the Raman on-resonance wave number
Photoswitchable SRS imaging of Drosophila larval fat bodies stained with DAE620 observed at the Raman off-resonance wave number