293-Kimata-PlantInhibit
Author reviewedNovel inhibitors of microtubule organization and phragmoplast formation in diverse plant species
Project ID
293-Kimata-PlantInhibit
Title
Novel inhibitors of microtubule organization and phragmoplast formation in diverse plant species
Description
The autors screened cell division-affecting compounds in Arabidopsis thaliana zygotes, whose cell division is traceable without time-lapse observations. They then determined the target events of the identified compounds using live-cell imaging of tobacco BY-2 cells. Subsequently, they isolated two compounds, PD-180970 and PP2, neither of which caused lethal damage. PD-180970 disrupted microtubule (MT) organization and, thus, nuclear separation, and PP2 blocked phragmoplast formation and impaired cytokinesis. Phosphoproteomic analysis showed that these compounds reduced the phosphorylation of diverse proteins, including MT-associated proteins (MAP70) and class II Kinesin-12.
Release Date
2024-12-14
Update Date
-
License
Version
1.0.0
Method Summary
See details in Kimata Y, et. al. Life Sci Alliance. 2023 Feb 27;6(5):e202201657.
Paper DOI
Kind
Image data
Organism
Arabidopsis thaliana
(
NCBITaxon:3702
)
Nicotiana tabacum
(
NCBITaxon:4097
)
Physcomitrium patens
(
NCBITaxon:3218
)
Strain
-
Cell line
-
Biological Process
cell division
(
GO:0051301
)
cytoskeleton organization
(
GO:0007010
)
Cellular Component
nucleus
(
GO:0005634
)
plasma membrane
(
GO:0005886
)
cytoskeleton
(
GO:0005856
)
phragmoplast
(
GO:0009524
)
Medical Subject Headings
-
Method involved in biological imaging
confocal microscopy
(
FBbi:00000251
)
time lapse microscopy
(
FBbi:00000249
)
two-photon laser scanning microscopy
(
FBbi:00000254
)
Image datasets
24
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Minako Ueda
Tohoku University
Graduate School of Life Sciences
-
-
Imaging dataset contributor
Yusuke Kimata
(Tohoku University)
Moe Yamada
(Nagoya University)
Takashi Murata
(Kanagawa Institute of Technology)
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse images of the Arabidopsis zygote expressing the histone and plasma membrane markers in the presence of DMSO.
Time-lapse images of the Arabidopsis zygote expressing the histone and plasma membrane markers in the presence of Oryzalin.
Time-lapse images of the Arabidopsis zygote expressing the histone and plasma membrane markers in the presence of PD-180970.
Time-lapse images of the Arabidopsis zygote expressing the histone and plasma membrane markers in the presence of PP2.
Time-lapse images of microtubule alignment in Arabidopsis zygote in the presence of DMSO.
Time-lapse images of microtubule alignment in Arabidopsis zygote in the presence of Oryzalin.
Time-lapse images of microtubule alignment in Arabidopsis zygote in the presence of PD-180970.
Time-lapse images of microtubule alignment in Arabidopsis zygote in the presence of PP2.
Time-lapse images of the BY-2 cells expressing PAKRP1 and microtubule marker in the presence of no compounds.
Time-lapse images of the BY-2 cells expressing PAKRP1 and microtubule marker in the presence of PP2.
Time-lapse images of P. patens chloronema tip cells expressing Pp-Kinesin-12IIc and microtubule marker in the presence of DMSO.
Time-lapse images of P. patens chloronema tip cells expressing Pp-Kinesin-12IIc and microtubule marker in the presence of PP2.
Time-lapse images of microtubules and histone markers in tobacco BY-GTRC cell strain in the presence of DMSO.
Time-lapse images of microtubules and histone markers in tobacco BY-GTRC cell strain in the presence of Oryzalin.
Time-lapse images of microtubules and histone markers in tobacco BY-GTRC cell strain in the presence of PD-180970.
Time-lapse images of microtublues and histone markers in tobacco BY-GTRC cell strain in the presence of PP2.
Time-lapse images of BY-2 cells expressing mClover-PAKRP1_Ser and histon marker in the absence of PP2.
Time-lapse images of BY-2 cells expressing mClover-PAKRP1_Ser and histon marker in the presence of PP2.
Time-lapse images of BY-2 cells expressing mClover-PAKRP1_Asp and histon marker in the absence of PP2.
Time-lapse images of BY-2 cells expressing mClover-PAKRP1_Asp and histon marker in the presence of PP2.
Time-lapse images of P. patens chloronema cells expressing microtubule and histone marker in the presence of DMSO.
Time-lapse images of P. patens chloronema cells expressing microtubule and histone marker in the presence of PP2.
Time-lapse images of the BY-2 cells expressing PAKRP1L and microtubule marker in the presence of PP2.
Time-lapse images of the BY-2 cells expressing PAKRP1L and microtubule marker in the presence of no compounds.