309-Fujioka-RamanProbe
Author reviewedDetection of multiple enzyme activities simultaneously in live cells by using activatable Raman probes
Project ID
309-Fujioka-RamanProbe
Title
Detection of multiple enzyme activities simultaneously in live cells by using activatable Raman probes
Description
Detection of multiple enzyme activities simultaneously in live cultured cells and in cells of Drosophila wing disc and fat body ex vivo by using activatable Raman probes
Release Date
2024-11-25
Update Date
-
License
Version
1.0.0
Method Summary
See details in Fujioka H, et. al. J Am Chem Soc. 2023 Apr 26;145(16):8871-8881.
Paper DOI
Kind
Image data
Organism
Homo sapiens
(
NCBITaxon:9606
)
Drosophila
(
NCBITaxon:7215
)
Strain
-
Cell line
Biological Process
-
Cellular Component
-
Medical Subject Headings
-
Method involved in biological imaging
stimulated Raman scattering (SRS) imaging
fluorescence microscopy
(
FBbi:00000246
)
Image datasets
16
Quantitative datasets
0
Paper DOI / Paper URL
OMERO.gallery
People
Contact
Mako Kamiya
Tokyo Institute of Technology
Department of Life Science and Technology
-
-
Imaging dataset contributor
-
Quantitative dataset contributor
-
Datasets
Thumbnail
Dataset
Organism
Kind / Links
SRS (stimulated Raman scattering) image of beta-galactosidase activity in live HEK-LacZ cells
SRS (stimulated Raman scattering) and fluorescence images of β-Gal, GGT, and DPP-4 activities in live A549 cells by using three kinds of Raman probes
SRS (stimulated Raman scattering) and fluorescence images of β-Gal, GGT, and DPP-4 activities in live H226 cells by using three kinds of Raman probes
Fluorescence image of membrane-located GFP and stimulated Raman scattering (SRS) image of bata- galactosidase activity in a Drosophila wing disc.
Fluorescence image of membrane and cytosolic GFP and stimulated Raman scattering (SRS) image of beta- galactosidase activity in Drosophila fat body
Confocal Stimulated Raman scattering (SRS) image of live HEK-LacZ cells using 9C15N-JCR-Bn-betaGal probe
Confocal Stimulated Raman scattering (SRS) image of live HEK-LacZ cells using 9CN-JR-Bn-betaGal probe
Confocal Stimulated Raman scattering (SRS) image of live HEK-LacZ cells treated with 9C15N-JCR-Bn-betaGal
Confocal Stimulated Raman scattering (SRS) image of beta-Gal activity in live cultured cells
Stimulated Raman scattering (SRS) image of beta-Gal activity in live HEK293 (without LacZ expression) cells
Stimulated Raman scattering (SRS) image of beta-galactosidase activity in live HEK-LacZ cells in the presence of a beta-galactosidase-specific inhibitor
The constructed 3D images of membrane located GFP and stimulated Raman scattering (SRS) image of beta-galactosidase activity in a Drosophila wing disc
Z-stack images of membrane located GFP and beta-galactosidase activity in a Drosophila wing disc
Fluorescence image of membrane-located GFP and dual-color SRS (stimulated Raman scattering) imaging of bata-galactosidase activity in Drosophila wing disc
Fluorescence image of membrane and cytosolic GFP and dual-color SRS (stimulated Raman scattering) imaging of beta-galactosidase activity in Drosophila fat body
Stimulated Raman scattering (SRS) image of GGT activity in Drosophila wing disc