SSBD:database

Thumbnail for 448-Temma-HeLaSPASIM

Summary of 448-Temma-HeLaSPASIM

SSBD:database
448-Temma-HeLaSPASIM
SSBD:database URL
https://ssbd.riken.jp/database/448-Temma-HeLaSPASIM/
SSBD:repository
ssbd-repos-000448
SSBD:repository URL
https://ssbd.riken.jp/repository/448/
Title
Selective-plane-activation structured illumination microscopy
Description
The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids. All the image data in this project is already registered at https://doi.org/10.5281/zenodo.10652642.
Release date
2026-07-07
Updated date
-
License
CC BY 4.0
Kind
Image data based on Experiment
Number of Datasets
40 ( Image datasets: 40, Quantitative data datasets: 0 )
Size of Datasets
8.7 GB ( Image datasets: 8.7 GB, Quantitative data datasets: 0 bytes )
Organism(s)
Mus musculus, Homo sapiens
Cell lines(s)
HeLa-S3 cell
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion, autophagosome, nucleus, actin filament
Biological Imaging Method
structured illumination microscopy (FBbi_00000332), wide-field microscopy, structured illumination microscopy (FBbi_00000332) time lapse microscopy (FBbi_00000249)
T scale
4 seconds, NA, 7 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Related paper(s)

Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita (2024) Selective-plane-activation structured illumination microscopy., Nature methods

Published in 2024 Apr 5 (Electronic publication in April 5, 2024, midnight )

(Abstract) The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.
Contact
Katsumasa Fujita , Osaka University , Department of Applied Physics , Department of Applied Physics
Contributors

Dataset List of 448-Temma-HeLaSPASIM

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#
Dataset ID
Kind
Size
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Thumbnail Thumbnail for dataset Fig2_onephoton_act_TL_SPASIM
# 13854
Datast ID Fig2_onephoton_act_TL_SPASIM
Dataset Kind Image data
Dataset Size 14.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_act_TL_SPIM
# 13855
Datast ID Fig2_onephoton_act_TL_SPIM
Dataset Kind Image data
Dataset Size 14.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_AP_SIM
# 13856
Datast ID Fig2_onephoton_AP_SIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_AP_SPASIM
# 13857
Datast ID Fig2_onephoton_AP_SPASIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_AP_SPIM
# 13858
Datast ID Fig2_onephoton_AP_SPIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_AP_WF
# 13859
Datast ID Fig2_onephoton_AP_WF
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_3D_3DSIM
# 13860
Datast ID Fig2_onephoton_mito_3D_3DSIM
Dataset Kind Image data
Dataset Size 1.3 GB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_3D_SPASIM
# 13861
Datast ID Fig2_onephoton_mito_3D_SPASIM
Dataset Kind Image data
Dataset Size 1.3 GB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_fixed_3DSIM
# 13862
Datast ID Fig2_onephoton_mito_fixed_3DSIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_fixed_SIM
# 13863
Datast ID Fig2_onephoton_mito_fixed_SIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_fixed_SPA3DSIM
# 13864
Datast ID Fig2_onephoton_mito_fixed_SPA3DSIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_fixed_SPASIM
# 13865
Datast ID Fig2_onephoton_mito_fixed_SPASIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_fixed_SPIM
# 13866
Datast ID Fig2_onephoton_mito_fixed_SPIM
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_fixed_WF
# 13867
Datast ID Fig2_onephoton_mito_fixed_WF
Dataset Kind Image data
Dataset Size 9.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_TL_SPASIM
# 13868
Datast ID Fig2_onephoton_mito_TL_SPASIM
Dataset Kind Image data
Dataset Size 134.5 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_onephoton_mito_TL_SPIM
# 13869
Datast ID Fig2_onephoton_mito_TL_SPIM
Dataset Kind Image data
Dataset Size 134.5 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_nulceus_3DSIM
# 13870
Datast ID Fig2_twophoton_H2B_nulceus_3DSIM
Dataset Kind Image data
Dataset Size 2.2 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_nulceus_SIM
# 13871
Datast ID Fig2_twophoton_H2B_nulceus_SIM
Dataset Kind Image data
Dataset Size 2.2 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_nulceus_SPA3DSIM
# 13872
Datast ID Fig2_twophoton_H2B_nulceus_SPA3DSIM
Dataset Kind Image data
Dataset Size 2.2 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_nulceus_SPASIM
# 13873
Datast ID Fig2_twophoton_H2B_nulceus_SPASIM
Dataset Kind Image data
Dataset Size 2.2 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_nulceus_SPIM
# 13874
Datast ID Fig2_twophoton_H2B_nulceus_SPIM
Dataset Kind Image data
Dataset Size 2.2 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_nulceus_WF
# 13875
Datast ID Fig2_twophoton_H2B_nulceus_WF
Dataset Kind Image data
Dataset Size 2.2 MB
4D view
SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_spheroid_3DSIM
# 13876
Datast ID Fig2_twophoton_H2B_spheroid_3DSIM
Dataset Kind Image data
Dataset Size 36.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_spheroid_SPA3DSIM
# 13877
Datast ID Fig2_twophoton_H2B_spheroid_SPA3DSIM
Dataset Kind Image data
Dataset Size 36.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_spheroid_SPIM
# 13878
Datast ID Fig2_twophoton_H2B_spheroid_SPIM
Dataset Kind Image data
Dataset Size 36.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig2_twophoton_H2B_spheroid_WF
# 13879
Datast ID Fig2_twophoton_H2B_spheroid_WF
Dataset Kind Image data
Dataset Size 36.0 MB
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Thumbnail Thumbnail for dataset Fig3_onephoton_mito_xy_SPASIM
# 13880
Datast ID Fig3_onephoton_mito_xy_SPASIM
Dataset Kind Image data
Dataset Size 18.2 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig3_twophoton_Lifeact_SPASIM_2D
# 13881
Datast ID Fig3_twophoton_Lifeact_SPASIM_2D
Dataset Kind Image data
Dataset Size 18.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig3_twophoton_Lifeact_SPASIM_3D
# 13882
Datast ID Fig3_twophoton_Lifeact_SPASIM_3D
Dataset Kind Image data
Dataset Size 1.8 GB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset Fig3_twophoton_spheroid_SPASIM_shifted
# 13883
Datast ID Fig3_twophoton_spheroid_SPASIM_shifted
Dataset Kind Image data
Dataset Size 2.8 GB
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Thumbnail Thumbnail for dataset FigS8_onephoton_mito2_SIM
# 13884
Datast ID FigS8_onephoton_mito2_SIM
Dataset Kind Image data
Dataset Size 404.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigS8_onephoton_mito2_SPASIM
# 13885
Datast ID FigS8_onephoton_mito2_SPASIM
Dataset Kind Image data
Dataset Size 400.0 MB
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Thumbnail Thumbnail for dataset FigS11_twophoton_act_rsG_3DSIM
# 13886
Datast ID FigS11_twophoton_act_rsG_3DSIM
Dataset Kind Image data
Dataset Size 36.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigS11_twophoton_act_rsG_SPASIM
# 13887
Datast ID FigS11_twophoton_act_rsG_SPASIM
Dataset Kind Image data
Dataset Size 36.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigS11_twophoton_act_rsG_SPIM
# 13888
Datast ID FigS11_twophoton_act_rsG_SPIM
Dataset Kind Image data
Dataset Size 36.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigS11_twophoton_act_rsG_WF
# 13889
Datast ID FigS11_twophoton_act_rsG_WF
Dataset Kind Image data
Dataset Size 16.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigS12_twophoton_nuclei_rsG_3DSIM
# 13890
Datast ID FigS12_twophoton_nuclei_rsG_3DSIM
Dataset Kind Image data
Dataset Size 36.0 MB
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SSBD:OMERO
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Thumbnail Thumbnail for dataset FigS12_twophoton_nuclei_rsG_SPASIM
# 13891
Datast ID FigS12_twophoton_nuclei_rsG_SPASIM
Dataset Kind Image data
Dataset Size 36.0 MB
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Thumbnail Thumbnail for dataset FigS12_twophoton_nuclei_rsG_SPIM
# 13892
Datast ID FigS12_twophoton_nuclei_rsG_SPIM
Dataset Kind Image data
Dataset Size 36.0 MB
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Thumbnail Thumbnail for dataset FigS12_twophoton_nuclei_rsG_WF
# 13893
Datast ID FigS12_twophoton_nuclei_rsG_WF
Dataset Kind Image data
Dataset Size 36.0 MB
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