Detail of Fig2_twophoton_H2B_spheroid_3DSIM
Project
SSBD:Repository
Title
Fluorescence images of spheroid of living HeLa cells labeled by rsGamillus-S in 3DSIM mode for two-photon activation
Description
Fluorescence images of histone H2B in the cell spheroids of living HeLa cells labeled by rsGamillus-S were obtained in three-dimensional structured illumination microscopy (3DSIM) mode using two-photon activation. The 3DSIM mode was difficult for imaging the internal structure of spheroid. rsGamillus-S is an acid-resistance photoswitchable fluorescent protein, which owns exceptionally high on-switching rate to show the nuclei more clearly. Two-photon activation using a scanned Bessel beam produces a wider FOV and a higher axial resolution.
Release, Updated
2026-07-07
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
36.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
nucleus
Biological Imaging Method
structured illumination microscopy (FBbi_00000332)
X scale
35.4 micrometer
Y scale
25400 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Temma K, Oketani R, Kubo T, Bando K, Maeda S, Sugiura K, Matsuda T, Heintzmann R, Kaminishi T, Fukuda K, Hamasaki M, Nagai T, Fujita K. Selective-plane-activation structured illumination microscopy. Nat Methods. 2024 May;21(5):889-896.
Related paper(s)
Contact
Katsumasa Fujita
, Osaka University
, Department of Applied Physics
, Department of Applied Physics
Contributors
OMERO Project
Source