Detail of Fig3_onephoton_mito_xy_SPASIM
Project
SSBD:Repository
Title
The x-y image of mitochondria of living HeLa cells in SPA-SIM mode for single-photon activation
Description
An x-y image of mitochondria in living HeLa cells visualized with Skylan-NS was captured using selective-plane-activation structured illumination microscopy (SPA-SIM) with single-photon activation. The x-y image shows the lateral distribution of fluorescence within mitochondria of living HeLa cells. Skylan-NS is a photoswitchable green fluorescent protein for improving super-resolution imaging for autophagosomes in living cells. A single-photon source is a light source that emits light as single particles or photons. The single-photon activation is single and with strong signal.
Release, Updated
2026-07-07
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
18.2 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion
Biological Imaging Method
structured illumination microscopy (FBbi_00000332)
X scale
100.7 micrometer
Y scale
100.7 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Temma K, Oketani R, Kubo T, Bando K, Maeda S, Sugiura K, Matsuda T, Heintzmann R, Kaminishi T, Fukuda K, Hamasaki M, Nagai T, Fujita K. Selective-plane-activation structured illumination microscopy. Nat Methods. 2024 May;21(5):889-896.
Related paper(s)
Contact
Katsumasa Fujita
, Osaka University
, Department of Applied Physics
, Department of Applied Physics
Contributors
OMERO Project
Source