Detail of Fig3_onephoton_mito_xy_SPASIM
Project
SSBD:Repository
Title
The x-y image of mitochondria of living HeLa cells in SPA-SIM mode for single-photon activation
Description
An x-y image of mitochondria in living HeLa cells visualized with Skylan-NS was captured using selective-plane-activation structured illumination microscopy (SPA-SIM) with single-photon activation. The x-y image shows the lateral distribution of fluorescence within mitochondria of living HeLa cells. Skylan-NS is a photoswitchable green fluorescent protein for improving super-resolution imaging for autophagosomes in living cells. A single-photon source is a light source that emits light as single particles or photons. The single-photon activation is single and with strong signal.
Release, Updated
2026-07-07
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
18.2 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion
Biological Imaging Method
structured illumination microscopy (FBbi_00000332)
X scale
100.7 micrometer
Y scale
100.7 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
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Microscope model
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Detector model
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Objective model
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Filter set
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Summary of Methods
Temma K, Oketani R, Kubo T, Bando K, Maeda S, Sugiura K, Matsuda T, Heintzmann R, Kaminishi T, Fukuda K, Hamasaki M, Nagai T, Fujita K. Selective-plane-activation structured illumination microscopy. Nat Methods. 2024 May;21(5):889-896.
Related paper(s)
Kenta Temma, Ryosuke Oketani, Toshiki Kubo, Kazuki Bando, Shunsuke Maeda, Kazunori Sugiura, Tomoki Matsuda, Rainer Heintzmann, Tatsuya Kaminishi, Koki Fukuda, Maho Hamasaki, Takeharu Nagai, Katsumasa Fujita (2024) Selective-plane-activation structured illumination microscopy., Nature methods
Published in 2024 Apr 5 (Electronic publication in April 5, 2024, midnight )
- doi: 10.1038/s41592-024-02236-3
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pmid: 38580844
(Abstract) The background light from out-of-focus planes hinders resolution enhancement in structured illumination microscopy when observing volumetric samples. Here we used selective plane illumination and reversibly photoswitchable fluorescent proteins to realize structured illumination within the focal plane and eliminate the out-of-focus background. Theoretical investigation of the imaging properties and experimental demonstrations show that selective plane activation is beneficial for imaging dense microstructures in cells and cell spheroids.
Contact
Katsumasa Fujita
, Osaka University
, Department of Applied Physics
, Department of Applied Physics
Contributors
OMERO Project
Source