Detail of FigS11_twophoton_act_rsG_SPASIM
Project
SSBD:Repository
Title
Fluorescence images of actin filaments in HeLa cell spheroids in SPA-SIM mode for two-photon activation
Description
Fluorescence images of actin filaments in living HeLa cell spheroids visualized with rsGamillus-S were obtained using selective-plane-activation structured illumination microscopy (SPA-SIM) with two-photon activation by a Bessel sheet. In the SPA-SIM system the aberrations make the imaging deeper parts difficult and limit the imaging depth to approximately 69 micrometre in a spheroid of ~200 micrometre in diameter. rsGamillus-S is an acid-resistance photoswitchable fluorescent protein, which owns exceptionally high on-switching rate to show the nuclei more clearly. Two-photon activation using a scanned Bessel beam produces a wider FOV and a higher axial resolution.
Release, Updated
2026-07-07
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
36.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
actin filament
Biological Imaging Method
structured illumination microscopy (FBbi_00000332)
X scale
141.8 micrometer
Y scale
141.8 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Temma K, Oketani R, Kubo T, Bando K, Maeda S, Sugiura K, Matsuda T, Heintzmann R, Kaminishi T, Fukuda K, Hamasaki M, Nagai T, Fujita K. Selective-plane-activation structured illumination microscopy. Nat Methods. 2024 May;21(5):889-896.
Related paper(s)
Contact
Katsumasa Fujita
, Osaka University
, Department of Applied Physics
, Department of Applied Physics
Contributors
OMERO Project
Source