Detail of Fig2_onephoton_mito_fixed_SPASIM
Project
SSBD:Repository
Title
Fluorescence images of mitochondria in fixed HeLa cells in the imaging mode of SPA-SIM for single-photon activation
Description
The fluorescence images of mitochondria labeled with Skylan-NS in fixed HeLa cells were taken and reconstructed in the imaging mode of selective-plane-activation structured illumination microscopy (SPA-SIM). The SPA-SIM mode produced large improvements in image contrast and spatial resolution, even for a densely labeled thick specimen. SPA-SIM reduced the influence of the out-of-focus background more effectively, which facilitates the visualization of intracellular structures. Skylan-NS is a photoswitchable green fluorescent protein. CW laser is used for single-photon activation, which is more efficient and allows measurement with higher temporal resolution.
Release, Updated
2026-07-07
License
CC BY 4.0
Kind
Image data
File Formats
tif
Data size
9.0 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
mitochondrion
Biological Imaging Method
structured illumination microscopy (FBbi_00000332)
X scale
70.9 micrometer
Y scale
70.9 micrometer
Z scale
-
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Temma K, Oketani R, Kubo T, Bando K, Maeda S, Sugiura K, Matsuda T, Heintzmann R, Kaminishi T, Fukuda K, Hamasaki M, Nagai T, Fujita K. Selective-plane-activation structured illumination microscopy. Nat Methods. 2024 May;21(5):889-896.
Related paper(s)
Contact
Katsumasa Fujita
, Osaka University
, Department of Applied Physics
, Department of Applied Physics
Contributors
OMERO Project
Source