484-Inagaki-SeeDBLive
Author reviewedImages of organoids, brain slices and in the brains of mice using minimally invasive optical clearing media, SeeDB-Live
Project ID
484-Inagaki-SeeDBLive
Project SID
484
Title
Images of organoids, brain slices and in the brains of mice using minimally invasive optical clearing media, SeeDB-Live
Description
Tissue clearing has been widely used for fluorescence imaging of fixed tissues, but its application to live tissues has been limited by toxicity. Here the authors develop minimally invasive optical clearing media for fluorescence imaging of live mammalian tissues. Light scattering is minimized by adding spherical polymers with low osmolarity to the extracellular medium. A clearing medium containing bovine serum albumin (SeeDB-Live) is compatible with live cells, enabling structural and functional imaging of live tissues, such as spheroids, organoids, acute brain slices and the mouse brains in vivo. SeeDB-Live minimally affects neuronal electrophysiological properties and sensory responses in vivo, and facilitates fluorescence imaging of deep cortical layers in live animals without detectable toxicity to neurons or behavior. They further demonstrate its utility to epifluorescence voltage imaging in acute brain slices and in vivo preparations. Thus, SeeDB-Live expands both the depth and modality range of fluorescence imaging in live mammalian tissues.
Submission Date
2025-11-25
Opened Date
-
Release Date
2026-08-19
Update Date
-
Kind
Image datasets
License
Project URL
-
Project DOI
-
Metadata Version
-
Template Version
3.03
Funding Information
This work was supported by grants from CREST program (JPMJCR2021 to T.I.), FOREST Program (JPMJFR230P to S.I.) of the Japan Science and Technology Agency (JST), AMED (JP23wm0525012 to T.I., JP25wm0625128 to T.I. and S.I., JP19dm0207080 to K.K., JP19dm0207079 to S.M., JP23gm6510022 and JP24wm0625119 to M.S.), the JSPS KAKENHI (JP21H00205, JP21H05696, JP23H02577, JP23H04236, JP23K18165, JP24H02308 and JP24H02312 to T.I., JP21H02140, JP22K18373 and JP24K01702 to S.I., JP19K06886 and JP24K02132 to S.F., JP22K06446, JP22H05094, JP24H01289 and JP25K02560 to N.N.-T., JP22H05161, JP22H00460 and JP23K18161 to K.K., JP22H02718 to S.M., JP24K18240 and JP25H02500 to T.Y., JP23K06151 to Y.K., JP20K23378 to T.K.S., JP24H00861 and JP25K21772 to M.S., JP25KJ1906 to H.T.), Kagoshima University Megumikai Medical Research Promotion Fund (to Y.T.), World Premier International Research Center Initiative (WPI-PRIMe; to K.H.), the Mochida Memorial Foundation for Medical and Pharmaceutical Research and the Uehara Memorial Foundation (to T.I.).
Total
166 files / 48.4 GB
Image datasets
zipped 83 files / 17.6 GB, raw image 83 files / 30.8 GB, total 166 files / 48.4 GB
Quantitative datasets
zipped 0 files / 0 bytes, raw bdml 0 files / 0 bytes, total 0 files / 0 bytes
Biosample
Organism
Mus musculus
(
NCBITaxon:10090
)
Strain
C57BL/6N
(
https://www.jax.org/strain/005304
)
Cell
neuron
mitral cell
(
CL:1001502
)
neuron
(
CL:0000540
)
neuron
extratelencephalic-projecting glutamatergic cortical neuron
(
CL:4023009
)
neuron
cerebral cortex pyramidal neuron
(
CL:4023111
)
Cell line
-
MeSH
Neurons
(
D009474
)
Microscopy, Fluorescence
(
D008856
)
Olfactory Bulb
(
D009830
)
Dendrites
(
D003712
)
Brain
(
D001921
)
Microscopy, Fluorescence, Multiphoton
(
D036641
)
Cell Body
(
D066146
)
Time-Lapse Imaging
(
D059008
)
Cerebral Cortex
(
D002540
)
Pyramidal Cells
(
D017966
)
Calcium Signaling
(
D020013
)
Cytoplasm
(
D003593
)
Microscopy, Confocal
(
D018613
)
Ontology
Anatomical Entity
-
UBERON
Biological Process
response to odorant
(
GO:1990834
)
neuronal action potential propagation
(
GO:0019227
)
neuronal action potential back-propagation
(
GO:0098873
)
calcium ion transport
(
GO:0006816
)
Cellular Component
Molecular Function
-
Medical Subject Headings
Neurons
(
D009474
)
Microscopy, Fluorescence
(
D008856
)
Olfactory Bulb
(
D009830
)
Dendrites
(
D003712
)
Brain
(
D001921
)
Microscopy, Fluorescence, Multiphoton
(
D036641
)
Cell Body
(
D066146
)
Time-Lapse Imaging
(
D059008
)
Cerebral Cortex
(
D002540
)
Pyramidal Cells
(
D017966
)
Calcium Signaling
(
D020013
)
Cytoplasm
(
D003593
)
Microscopy, Confocal
(
D018613
)
Imaging Method
Method involved in biological imaging
fluorescence microscopy
(
FBbi:00000246
)
epifluorescence microscopy
two-photon laser scanning microscopy
(
FBbi:00000254
)
time lapse microscopy
(
FBbi:00000249
)
confocal microscopy
(
FBbi:00000251
)
Paper DOI / Paper URL
People
Contact
Shigenori Inagaki
Kyushu University
Department of Developmental Neurophysiology
-
-
Takeshi Imai
Kyushu University
Department of Developmental Neurophysiology
-
-
Imaging dataset contributor
Shigenori Inagaki (Kyushu University)
Nathan Zechen Huynh (Kagoshima University)
Yuki Kambe (Kagoshima University)
Takahiro Noda (Kyushu University)
Hikari Takeshima (Kyushu University)
Tatsushi Yokoyama (Kyoto University)
Masayuki Sakamoto (Kyoto University)
Tatsuo K. Sato (Kagoshima University)
Satoshi Manita (University of Yamanashi)
Kazuo Kitamura (University of Yamanashi)
Quantitative dataset contributor
-
Image datasets
83
Quantitative datasets
0
Dataset List
Thumbnail
Dataset
Organism
Kind / Links
Time-lapse voltage imaging of glomerular layer of the olfactory bulb in vivo using SeeDB-Live
Two-photon voltage imaging of JEDI-2P-labelled L2/3 neurons in S1 of awake mice
Two-photon voltage imaging of JEDI-2P-labelled L5 neurons in S1 of awake mice
Z-series images of mitral cells in the olfactory bulb slice expressing GCaMP6f
Time-lapse images of mitral cells in the olfactory bulb slice expressing Voltron2
Time-lapse images of Voltron2-ST labelled L2/3 neurons in S1 of anesthetized mice after clearing with SeeDB-Live
Mus musculus
(
NCBITaxon:10090
)
Time-lapse images of Voltron2-ST labelled L2/3 neurons in S1 of anesthetized mice before clearing with SeeDB-Live
Mus musculus
(
NCBITaxon:10090
)
Z-series images of mTurquoise2 labelled L2/3 neurons in S1 of awake mouse
Mus musculus
(
NCBITaxon:10090
)
Time-lapse images of Voltron2 labelled L2/3 neurons in S1 of awake mouse
Time-lapse calcium imaging of cortical organoid using SeeDB-Live
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Time-lapse calcium imaging of mouse olfactory bulb slice after washing out SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice before immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice after washing out SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice after washing out SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice after washing out SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice after washing out SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice before immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice before immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice before immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice before immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice during immersing in SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb slice in ACSF
Time-lapse calcium imaging of mouse olfactory bulb slice in SeeDB-Live
Z-series images of mouse cerebral cortex in vivo using SeeDB-Live
Z-series images of mouse cerebral cortex in vivo using ACSF-HEPES
Z-series images of mouse cerebral cortex in vivo using SeeDB-Live
Z-series images of mouse cerebral cortex in vivo using SeeDB-Live
Z-series images of mouse cerebral cortex in vivo using SeeDB-Live
Z-series images of mouse cerebral cortex in vivo using ACSF-HEPES
Z-series images of mouse cerebral cortex in vivo using ACSF-HEPES
Z-series images of mouse cerebral cortex in vivo using ACSF-HEPES
Time-lapse calcium imaging of mouse olfactory bulb in vivo using SeeDB-Live
Time-lapse calcium imaging of mouse olfactory bulb in vivo using ACSF-HEPES
Time-lapse calcium imaging of L5ET neurons in primary somatosensory
cortex using SeeDB-Live
Time-lapse calcium imaging of L5ET neurons in primary somatosensory
cortex using SeeDB-Live
Time-lapse calcium imaging of L5ET neurons in primary somatosensory
cortex using ACSF-HEPES
Time-lapse calcium imaging of L5ET neurons in primary somatosensory
cortex using ACSF-HEPES
Time-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory
cortex at Day100
Time-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory
cortex at Day120
Time-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory
cortex at Day7
Time-lapse calcium imaging of layer 2 and 3 neurons in primary somatosensory
cortex at Day80
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using SeeDB-Live
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using ACSF
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using SeeDB-Live
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using ACSF
Z-series images of mitral cells in the olfactory bulb slice expressing GCaMP6f
Time-lapse images of mitral cells in the olfactory bulb slice expressing Voltron2
Time-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 after clearing with SeeDB-Live
Time-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 before clearing with SeeDB-Live
Time-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 after clearing with SeeDB-Live