SSBD:database

484-Inagaki-SeeDBLive/Fig6d_2P_GCaMP6f thumbnail

484-Inagaki-SeeDBLive/Fig6d_2P_GCaMP6f

Author reviewed
Z-series images of mitral cells in the olfactory bulb slice expressing GCaMP6f
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Project
484-Inagaki-SeeDBLive
Dataset ID
Fig6d_2P_GCaMP6f
Kind
Image data
Title
Z-series images of mitral cells in the olfactory bulb slice expressing GCaMP6f
Description
Z-series images of GCaMP6f-expressing mitral cells in mouse olfactory bulb slice at P11. Voltron2, a fast and sensitive chemigenetic voltage indicator, and GCaMP6f were sparsely introduced to mitral/tufted cells by in utero electroporation. The slice in SeeDB-Live was imaged by two-photon confocal microscopy. This dataset includes the images of GCaMP6f.
Folder Name
Fig6d_2P_GCaMP6f
File Format
.tif
File Size
33MB
File Separation Method
-
Figure Number
Fig6d
Dimensions
512x512x65x1x1
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
3 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-

Biosample

Description
Mouse olfactory bulb slice at P11 in which Voltron2 plasmid and GCaMP6f plasmid were sparsely expressed in mitral cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
pCAG-GCaMP6f plasmid and pGP-pcDNA3.1 Puro-CAG-Voltron2 plasmid were introduced into the E12 brain by in utero electroporation. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated with the slice before imaging.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
-
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-