484-Inagaki-SeeDBLive/Fig4f_L5ET_ctrl_2
Author reviewedZ-series images of mouse cerebral cortex in vivo using ACSF-HEPES
Project
Dataset ID
Fig4f_L5ET_ctrl_2
Kind
Image data
Title
Z-series images of mouse cerebral cortex in vivo using ACSF-HEPES
Description
Z-series images of anesthetized mouse cerebral cortex using ACSF-HEPES. The neurons at a depth of 0-700 micrometer from the surface of the brain were imaged by two-photon confocal microscopy. EYFP expressed in the layer 5 extratelencephalic-projecting (L5ET) neurons were observed in the Thy-YFP-H transgenic mouse.
Folder Name
Fig4f_L5ET_ctrl_2
File Format
.tif
File Size
707MB
File Separation Method
-
Figure Number
Fig4f
Dimensions
1024x1024x337x1x1
X Scale
0.520 micrometer
Y Scale
0.520 micrometer
Z Scale
3 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
An anesthetized Thy-YFP-H transgenic mouse. Craniotomy and durotomy were made on the right hemisphere. The brain surface was perfused with ACSF-HEPES and perfused for 1 h under anesthesia.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron
extratelencephalic-projecting glutamatergic cortical neuron (CL:4023009)
Cell line
-
MeSH
Intrinsic Variable
Transgenic mouse, Thy-YFP-H, in which L5ET neurons are labeled with EYFP.
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
-
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / B6.Cg-Tg(Thy1-YFP)HJrs/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
Yellow fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Thorlabs MM201
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-