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484-Inagaki-SeeDBLive/Fig6b_voltageMT_ctrl thumbnail

484-Inagaki-SeeDBLive/Fig6b_voltageMT_ctrl

Author reviewed
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using ACSF
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Project
484-Inagaki-SeeDBLive
Dataset ID
Fig6b_voltageMT_ctrl
Kind
Image data
Title
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using ACSF
Description
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice at P11. Voltron2, a fast and sensitive chemigenetic voltage indicator, was sparsely introduced to mitral/tufted cells by in utero electroporation. The slice in ACSF was imaged by epifluorescence microscopy.
Folder Name
Fig6b_voltageMT_ctrl
File Format
.tif
File Size
259MB
File Separation Method
-
Figure Number
Fig6b
Dimensions
376x252x1x1x1365
X Scale
1.482 micrometer
Y Scale
1.482 micrometer
Z Scale
-
T Scale
-
Channel
1 channel
Quantitative Data
-

Biosample

Description
Mouse olfactory bulb slice at P11 in which Voltron2 plasmid was sparsely expressed in mitral cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
pGP-pcDNA3.1 Puro-CAG-Voltron2 plasmid was introduced into the E12 brain by in utero electroporation. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated with the slice before imaging.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuronal action potential propagation (GO:0019227)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
epifluorescence microscopy
Detection Method
high-speed CMOS camera
Visualization Method
Voltron2 was visualized with JF549–HaloTag ligand applied to the medium (Voltron2_{549})
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-