SSBD:database

484-Inagaki-SeeDBLive/Fig3jk_NMDAstim_ctrl_2 thumbnail

484-Inagaki-SeeDBLive/Fig3jk_NMDAstim_ctrl_2

Author reviewed
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
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Project
484-Inagaki-SeeDBLive
Dataset ID
Fig3jk_NMDAstim_ctrl_2
Kind
Image data
Title
Time-lapse calcium imaging of mouse olfactory bulb stimulated with NMDA
Description
Time-lapse calcium imaging of mouse olfactory bulb slice immersed in ACSF. In the mitral cells of the olfactory bulb, GCaMP6f was expressed. Activities of the neurons stimulated with NMDA and glycine at a depth of 100 micrometer from the surface of the slice were imaged. This dataset includes the images before, during and after washing out the stimulant.
Folder Name
Fig3jk_NMDAstim_ctrl_2
File Format
.tif
File Size
174MB
File Separation Method
-
Figure Number
Fig3jk
Dimensions
512x512x1x1x332
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
-
T Scale
1.109 seconds
Channel
1 channel
Quantitative Data
-

Biosample

Description
An acute olfactory bulb slice from mouse at postnatal day 15, which was immersed in ACSF. GCaMP6f was expressed in mitral cells of the olfactory bulb.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f in which GCaMP6f is expressed in neurons.
Extrinsic Variable
The olfactory bulb slice was treated with NMDA and glycine during imaging

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
cell body (GO:0044297)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
N-methyl-D-aspartic acid
Concentration
100 micromolar
Fold Dilution
-
Reagent / Compound
glycine
Concentration
40 micromolar
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-