484-Inagaki-SeeDBLive/Fig2d_1p_ctrl_2
Author reviewedZ-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Project
Dataset ID
Fig2d_1p_ctrl_2
Kind
Image data
Title
Z-series images of L5ET neurons in primary somatosensory
cortex of Thy1-YFP-H mouse
Description
Z-series images of an acute brain slice (300-micrometer thick) of Thy1-YFP-H mouse, in which layer 5 extratelencephalic-projecting (L5ET) neurons of somatosensory cortex were visualized. The brain slice in control ACSF was imaged by one-photon confocal microscopy.
Folder Name
Fig2d_1p_ctrl_2
File Format
.tif
File Size
34MB
File Separation Method
-
Figure Number
Fig2d
Dimensions
512x512x67x1x1
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
3 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-
Biosample
Description
An acute brain slice
(300-μm thick) from Thy1-YFP-H mouse at postnatal day 22, which was immersed in ACSF.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron
extratelencephalic-projecting glutamatergic cortical neuron (CL:4023009)
Cell line
-
MeSH
Intrinsic Variable
Transgenic mouse (Thy1-YFP-H mouse) that neurons in the brain express EYFP
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
-
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / B6.Cg-Tg(Thy1-YFP)HJrs/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
Detection Method
-
Visualization Method
Yellow fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-