SSBD:database

484-Inagaki-SeeDBLive/ExtFig9abc_SliceVoltage_voltron2 thumbnail

484-Inagaki-SeeDBLive/ExtFig9abc_SliceVoltage_voltron2

Author reviewed
Time-lapse images of mitral cells in the olfactory bulb slice expressing Voltron2
SSBD:OMERO.gallery (Project) SSBD:OMERO (Project) SSBD:OMERO.gallery (Dataset) SSBD:OMERO (Dataset)
SSBD:downloads
Project
484-Inagaki-SeeDBLive
Dataset ID
ExtFig9abc_SliceVoltage_voltron2
Kind
Image data
Title
Time-lapse images of mitral cells in the olfactory bulb slice expressing Voltron2
Description
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice at P8. Voltron2, a fast and sensitive chemigenetic voltage indicator, and GCaMP6f were sparsely introduced to mitral/tufted cells by in utero electroporation. The slice in SeeDB-Live was imaged by epifluorescence microscopy. This dataset includes the images of Voltron2_{549}.
Folder Name
ExtFig9abc_SliceVoltage_voltron2
File Format
.tif
File Size
330MB
File Separation Method
-
Figure Number
Extended Data Fig9
Dimensions
128x128x1x1x10000
X Scale
2.89 micrometer
Y Scale
2.89 micrometer
Z Scale
-
T Scale
0.5 milliseconds
Channel
1 channel
Quantitative Data
-

Biosample

Description
Mouse olfactory bulb slice at P8 in which Voltron2 plasmid and GCaMP6f plasmid were sparsely expressed in mitral cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
pCAG-GCaMP6f plasmid and pGP-pcDNA3.1 Puro-CAG-Voltron2 plasmid were introduced into the E12 brain by in utero electroporation. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated with the slice before imaging.

Ontology

Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
neuronal action potential propagation (GO:0019227)
neuronal action potential back-propagation (GO:0098873)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
time lapse microscopy (FBbi:00000249)
epifluorescence microscopy
Detection Method
high-speed CMOS camera
Visualization Method
Voltron2 was visualized with JF549–HaloTag ligand applied to the medium (Voltron2_{549})
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue tissue clearing
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Brain (D001921)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Cerebral Cortex (D002540)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-