SSBD:database

Project

Dataset ID

Fig5k_awakeL5_ctrl_day0

Kind

Image data

Title

Time-lapse calcium imaging of L5ET neurons in primary somatosensory cortex using ACSF-HEPES

Description

Time-lape images of awake mouse cerebral cortex using ACSF-HEPES. The neurons at a depth of 615 micrometer from the surface of the brain were imaged by two-photon confocal microscopy. jGCaMP8m expressed in the layer 5 extratelencephalic-projecting (L5ET) neurons were observed in the mouse that was injected AAV-jGCaMP8m-P2A-CyRFP1. The brain surface was perfused with ACSF-HEPES. The mouse was imaged two times at conscutive days. This is the imaging data at the first day. 2 channels are stacked into a single file. 1-501 frames for jGCaMP8m, 502-1002 frames for CyRFP1.

Folder Name

Fig5k_awakeL5_ctrl_day0

File Format

.tif

File Size

527MB

File Separation Method

2 channels are stacked into a single file. 1-501 frames for jGCaMP8m, 502-1002 frames for CyRFP1

Figure Number

Fig5k

Dimensions

512x512x1x2x501

X Scale

1.043 micrometer

Y Scale

1.043 micrometer

Z Scale

-

T Scale

0.169 seconds

Channel

2 channel

Quantitative Data

-

Description

An awake mouse injected AAV-jGCaMP8m-P2A-CyRFP1. jGCaMP8m and CyRFP1 was expressed in L5ET neurons in primary somatosensory cortex. Craniotomy and durotomy were made on the right cortex. The brain surface was perfused with ACSF-HEPES.

Organism

Strain

-

Cell

neuron extratelencephalic-projecting glutamatergic cortical neuron (CL:4023009)

Cell line

-

MeSH

Intrinsic Variable

-

Extrinsic Variable

L5 neurons in S1 were labeled with AAV-jGCaMP8m-P2A-CyRFP1.

Anatomical Entity

-

UBERON

Biological Process

Cellular Component

Molecular Function

-

MeSH Anatomy

MeSH BP

MeSH CC

MeSH MF

Imaging Method

Detection Method

-

Visualization Method

jGCaMP8m CyRFP1

Illumination Method

-

Source of Contrast

-

Imaged Parameter

fluorescence emission

Sample Preparation Method

living tissue

MeSH

Body

Thorlabs MM201

Module

-

Light Source

-

Detector

-

Objective

Olympus-Evident XLPLN25XWMP

Filter Set

-

Dichroic

-