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484-Inagaki-SeeDBLive/Fig2e_2p_ctrl_5 thumbnail

484-Inagaki-SeeDBLive/Fig2e_2p_ctrl_5

Author reviewed
Z-series images of L5ET neurons in primary somatosensory cortex of Thy1-YFP-H mouse
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Project
484-Inagaki-SeeDBLive
Dataset ID
Fig2e_2p_ctrl_5
Kind
Image data
Title
Z-series images of L5ET neurons in primary somatosensory cortex of Thy1-YFP-H mouse
Description
Z-series images of an acute brain slice (300-micrometer thick) of Thy1-YFP-H mouse, in which layer 5 extratelencephalic-projecting (L5ET) neurons of somatosensory cortex were visualized. The brain slice in control ACSF was imaged by two-photon confocal microscopy.
Folder Name
Fig2e_2p_ctrl_5
File Format
.tif
File Size
51MB
File Separation Method
-
Figure Number
Fig2e
Dimensions
512x512x101x1x1
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
3 micrometer
T Scale
-
Channel
1 channel
Quantitative Data
-

Biosample

Description
An acute brain slice (300-μm thick) from Thy1-YFP-H mouse at postnatal day 22, which was immersed in ACSF.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron extratelencephalic-projecting glutamatergic cortical neuron (CL:4023009)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Cerebral Cortex (D002540)
Dendrites (D003712)
Intrinsic Variable
Transgenic mouse (Thy1-YFP-H mouse) that neurons in the brain express EYFP
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
brain (UBERON:0000955)
cerebral cortex (UBERON:0000956)
Biological Process
-
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Cerebral Cortex (D002540)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Cerebral Cortex (D002540)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / B6.Cg-Tg(Thy1-YFP)HJrs/J

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
Detection Method
-
Visualization Method
Yellow fluorescent protein from Aequorea
Illumination Method
-
Source of Contrast
distribution of a specific protein
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Brain (D001921)
Cell Body (D066146)
Pyramidal Cells (D017966)
Cerebral Cortex (D002540)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-