SSBD:database

484-Inagaki-SeeDBLive/Fig6c_voltageMT_ctrl thumbnail

484-Inagaki-SeeDBLive/Fig6c_voltageMT_ctrl

Author reviewed
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using ACSF
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Project
484-Inagaki-SeeDBLive
Dataset ID
Fig6c_voltageMT_ctrl
Kind
Image data
Title
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using ACSF
Description
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice at P11. Voltron2, a fast and sensitive chemigenetic voltage indicator, was sparsely introduced to mitral/tufted cells by in utero electroporation. Neurons at 150 micrometer depth of the slice in ACSF was imaged by epifluorescence microscopy.
Folder Name
Fig6c_voltageMT_ctrl
File Format
.tif
File Size
259MB
File Separation Method
-
Figure Number
Fig6c
Dimensions
376x252x1x1x1365
X Scale
1.482 micrometer
Y Scale
1.482 micrometer
Z Scale
-
T Scale
7 milliseconds
Channel
1 channel
Quantitative Data
-

Biosample

Description
Mouse olfactory bulb slice at P11 in which Voltron2 plasmid was sparsely expressed in mitral cells.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
Intrinsic Variable
-
Extrinsic Variable
pGP-pcDNA3.1 Puro-CAG-Voltron2 plasmid was introduced into the E12 brain by in utero electroporation. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated with the slice before imaging.

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuronal action potential propagation (GO:0019227)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transfection electroporation

Treatments

Reagent / Compound
-
Concentration
-
Fold Dilution
-

Imaging Method

Imaging Method
fluorescence microscopy (FBbi:00000246)
time lapse microscopy (FBbi:00000249)
epifluorescence microscopy
Detection Method
high-speed CMOS camera
Visualization Method
Voltron2 was visualized with JF549-HaloTag ligand applied to the medium (Voltron2_{549})
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence (D008856)
Olfactory Bulb (D009830)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Dendrites (D003712)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-