SSBD:database

484-Inagaki-SeeDBLive/Fig5f_odor_ctrl thumbnail

484-Inagaki-SeeDBLive/Fig5f_odor_ctrl

Author reviewed
Time-lapse calcium imaging of mouse olfactory bulb in vivo using ACSF-HEPES
SSBD:OMERO.gallery (Project) SSBD:OMERO (Project) SSBD:OMERO.gallery (Dataset) SSBD:OMERO (Dataset)
SSBD:downloads
Project
484-Inagaki-SeeDBLive
Dataset ID
Fig5f_odor_ctrl
Kind
Image data
Title
Time-lapse calcium imaging of mouse olfactory bulb in vivo using ACSF-HEPES
Description
Odor responses of mitral cells of the olfactory bulb in anesthetized mouse. In the mitral cells, GCaMP6f was expressed. Activities of the mitral cell in response to odor stimulation (1% valeraldehyde) at a depth of 381 micrometer from the surface of the brain were imaged. The odor was delivered to a mouse nose for 5 seconds at 1 litter per minute. The brain surface was perfused with ACSF-HEPES.
Folder Name
Fig5f_odor_ctrl
File Format
.tif
File Size
11.7MB
File Separation Method
-
Figure Number
Fig5f
Dimensions
512x512x1x1x22
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
-
T Scale
1.644 seconds
Channel
1 channel
Quantitative Data
-

Biosample

Description
An anesthetized Thy1-GCaMP6f mouse (2- to 4-month-old, male and female). GCaMP6f was expressed in mitral cells of the olfactory bulb. Craniotomy and durotomy were made on the right olfactory bulb. The brain surface was perfused with ACSF-HEPES.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron mitral cell (CL:1001502)
Cell line
-
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
Intrinsic Variable
A mouse line Thy1-GCaMP6f in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-

Ontology

Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
response to odorant (GO:1990834)
Cellular Component
cell body (GO:0044297)
Molecular Function
-
MeSH Anatomy
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH BP
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH CC
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)
MeSH MF
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Genetics

Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J

Treatments

Reagent / Compound
pentanal
Concentration
1 percent
Fold Dilution
-

Imaging Method

Imaging Method
two-photon laser scanning microscopy (FBbi:00000254)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Neurons (D009474)
Microscopy, Fluorescence, Multiphoton (D036641)
Olfactory Bulb (D009830)
Calcium Signaling (D020013)
Cell Body (D066146)
Time-Lapse Imaging (D059008)
Brain (D001921)

Instrument

Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-