484-Inagaki-SeeDBLive/Fig3o_P13_ACSF
Author reviewedTime-lapse calcium imaging of mouse olfactory bulb slice in ACSF
Project
Dataset ID
Fig3o_P13_ACSF
Kind
Image data
Title
Time-lapse calcium imaging of mouse olfactory bulb slice in ACSF
Description
Time-lapse calcium imaging of mouse olfactory bulb slice in ACSF. GCaMP6f was expressed in the mitral cells of the olfactory bulb, and it was imaged by confocal microscopy. Spontaneous activities of the neurons at a depth of 100 micrometer from the surface of the slice were imaged.
Folder Name
Fig3o_P13_ACSF
File Format
.tif
File Size
16MB
File Separation Method
-
Figure Number
Fig3o
Dimensions
363x363x1x1x60
X Scale
0.994 micrometer
Y Scale
0.994 micrometer
Z Scale
-
T Scale
1.109 seconds
Channel
1 channel
Quantitative Data
-
Paper(s)
Biosample
Description
An acute olfactory bulb slice from mouse at postnatal day 13, which was immersed in ACSF. GCaMP6f was expressed in mitral cells of the olfactory bulb.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
A mouse line Thy1-GCaMP6f in which GCaMP6f is expressed in neurons.
Extrinsic Variable
-
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
calcium ion transport (GO:0006816)
Cellular Component
cell body (GO:0044297)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic / C57BL/6J-Tg(Thy1-GCaMP6f)GP5.11Dkim/J
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
confocal microscopy (FBbi:00000251)
time lapse microscopy (FBbi:00000249)
Detection Method
-
Visualization Method
GCaMP6f
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-