484-Inagaki-SeeDBLive/Fig6hi_voltageOB_ctrl
Author reviewedTime-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 before clearing with SeeDB-Live
Project
Dataset ID
Fig6hi_voltageOB_ctrl
Kind
Image data
Title
Time-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 before clearing with SeeDB-Live
Description
Time-lapse images of mitral cells expressing Voltron2 in the olfactory bulb of an anesthetized mouse before clearing with SeeDB-Live. Voltron2 was specifically and sparsely expressed in mitral/tufted cells using the Pcdh21-Cre driver and a Cre-dependent adeno-associated virus (AAV) vector, labeled with JF549-HaloTag ligand (Voltron2_{549}). Glomerular layer of the olfactory bulb (90 micrometer depth of the slice) was imaged.
Folder Name
Fig6hi_voltageOB_ctrl
File Format
.tif
File Size
94MB
File Separation Method
-
Figure Number
Fig6hi
Dimensions
256x256x1x1x715
X Scale
2.89 micrometer
Y Scale
2.89 micrometer
Z Scale
-
T Scale
7 milliseconds
Channel
1 channel
Quantitative Data
-
Biosample
Description
An anesthetized Voltron2-expressing transgenic mouse (4-month-old). Craniotomy and durotomy were made on the right hemisphere. The brain surface was perfused with ACSF-HEPES and perfused for 1 h under anesthesia.
Organism
Mus musculus (NCBITaxon:10090)
Strain
-
Cell
neuron
mitral cell (CL:1001502)
Cell line
-
MeSH
Intrinsic Variable
Pcdh21-Cre transgennic mouse was used.
Extrinsic Variable
To express Volton2 by Cre dependent manner, AAV-syn-FLEX-Volton2 was injected into the olfactory bulb of Pcdh21-Cre mouse. 50 nM Janelia Fluor HaloTag Ligand 549 was incubated with the slice before imaging.
Ontology
Anatomical Entity
-
UBERON
olfactory bulb brain (UBERON:0000955)
Biological Process
neuronal action potential propagation (GO:0019227)
Cellular Component
cell body (GO:0044297)
dendrite (GO:0030425)
Molecular Function
-
MeSH Anatomy
MeSH BP
MeSH CC
MeSH MF
Genetics
Gene
-
Protein / Probe
-
Method / Genotype
transgenic
AAV-vector injection / Pcdh21-Cre
Treatments
Reagent / Compound
-
Concentration
-
Fold Dilution
-
Imaging Method
Imaging Method
fluorescence microscopy (FBbi:00000246)
epifluorescence microscopy
Detection Method
high-speed CMOS
camera
Visualization Method
Voltron2 was visualized with JF549-HaloTag
ligand applied to the medium (Voltron2_{549})
Illumination Method
-
Source of Contrast
-
Imaged Parameter
fluorescence emission
Sample Preparation Method
living tissue
tissue clearing
MeSH
Instrument
Body
Olympus-Evident FV1000MPE
Module
-
Light Source
-
Detector
-
Objective
Olympus-Evident XLPLN25XWMP
Filter Set
-
Dichroic
-