SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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484-Inagaki-SeeDBLive

https://ssbd.riken.jp/database/484-Inagaki-SeeDBLive/

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ssbd-repos-000484

https://ssbd.riken.jp/repository/484/

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Images of organoids, brain slices and in the brains of mice using minimally invasive optical clearing media, SeeDB-Live

Tissue clearing has been widely used for fluorescence imaging of fixed tissues, but its application to live tissues has been limited by toxicity. Here the authors develop minimally invasive optical clearing media for fluorescence imaging of live mammalian tissues. Light scattering is minimized by adding spherical polymers with low osmolarity to the extracellular medium. A clearing medium containing bovine serum albumin (SeeDB-Live) is compatible with live cells, enabling structural and functional imaging of live tissues, such as spheroids, organoids, acute brain slices and the mouse brains in vivo. SeeDB-Live minimally affects neuronal electrophysiological properties and sensory responses in vivo, and facilitates fluorescence imaging of deep cortical layers in live animals without detectable toxicity to neurons or behavior. They further demonstrate its utility to epifluorescence voltage imaging in acute brain slices and in vivo preparations. Thus, SeeDB-Live expands both the depth and modality range of fluorescence imaging in live mammalian tissues.

2026-08-19

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CC BY 4.0

Image data based on Experiment

83 ( Image datasets: 83, Quantitative data datasets: 0 )

30.8 GB ( Image datasets: 30.8 GB, Quantitative data datasets: 0 bytes )

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Mus musculus

C57BL/6N

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neuronal action potential propagation, calcium ion transport, calcium ion transport response to odorant, neuronal action potential propagation neuronal action potential back-propagation, response to odorant neuronal action potential propagation neuronal action potential back-propagation

cell body, cytoplasm, cell body dendrite, dendrite

fluorescence microscopy, two-photon laser scanning microscopy, confocal microscopy

1.644 seconds, 0.405 seconds, 1.109 seconds, NA, 0.5 milliseconds, 0.169 seconds, 4 milliseconds, 8.8 milliseconds, 1 milliseconds, 1.115 seconds, 0.235 seconds, 1.110 seconds, 7 milliseconds

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Shigenori Inagaki, Nao Nakagawa-Tamagawa, Nathan Zechen Huynh, Yuki Kambe, Rei Yagasaki, Satoshi Manita, Satoshi Fujimoto, Takahiro Noda, Misato Mori, Aki Teranishi, Hikari Takeshima, Koki Ishikawa, Yuki Naitou, Tatsushi Yokoyama, Masayuki Sakamoto, Katsuhiko Hayashi, Kazuo Kitamura, Yoshiaki Tagawa, Satoru Okuda, Tatsuo K Sato, Takeshi Imai (2026) Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo., Nature methods

Published in March 12, 2026 (Electronic publication in March 12, 2026, midnight )

• doi: 10.1038/s41592-026-03023-y
• pmid: 41820664
  

(Abstract) Tissue clearing has been widely used for fluorescence imaging of fixed tissues, but its application to live tissues has been limited by toxicity. Here we develop minimally invasive optical clearing media for fluorescence imaging of live mammalian tissues. Light scattering is minimized by adding spherical polymers with low osmolarity to the extracellular medium. A clearing medium containing bovine serum albumin (SeeDB-Live) is compatible with live cells, enabling structural and functional imaging of live tissues, such as spheroids, organoids, acute brain slices and the mouse brains in vivo. SeeDB-Live minimally affects neuronal electrophysiological properties and sensory responses in vivo, and facilitates fluorescence imaging of deep cortical layers in live animals without detectable toxicity to neurons or behavior. We further demonstrate its utility to epifluorescence voltage imaging in acute brain slices and in vivo preparations. Thus, SeeDB-Live expands both the depth and modality range of fluorescence imaging in live mammalian tissues.

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Shigenori Inagaki, Takeshi Imai , Kyushu University, Kyushu University , Department of Developmental Neurophysiology, Department of Developmental Neurophysiology , Department of Developmental Neurophysiology, Department of Developmental Neurophysiology

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