Detail of ExtFig9hi_InVivoVoltage_awake_voltron2


Project
SSBD:Repository
Title
Time-lapse images of Voltron2 labelled L2/3 neurons in S1 of awake mouse
Description
Time-lapse images of mTurquoise2-expressing layer 2/3 neurons of somatosensory cortex in awake mouse. mTurquoise2 and Voltron2-ST were sparsely introduced into L2/3 neurons by in utero electroporation. The brain surface was perfused with SeeDB-Live during imaging. This dataset includes the images of Voltron2-ST.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
1.4 GB

Organism
Mus musculus ( NCBI:txid10090 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuronal action potential propagation neuronal action potential back-propagation
Cellular Component (CC)
dendrite
Biological Imaging Method
fluorescence microscopy ( Fbbi:00000246 )
X scale
2.89 micrometer
Y scale
2.89 micrometer
Z scale
-
T scale
4 milliseconds

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)

Contact
Shigenori Inagaki, Takeshi Imai , Kyushu University, Kyushu University , Department of Developmental Neurophysiology, Department of Developmental Neurophysiology , Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors

OMERO Dataset
OMERO Project
Source