Detail of Fig5k_awakeL5_ctrl_day0
Project
SSBD:Repository
Title
Time-lapse calcium imaging of L5ET neurons in primary somatosensory cortex using ACSF-HEPES
Description
Time-lape images of awake mouse cerebral cortex using ACSF-HEPES. The neurons at a depth of 615 micrometer from the surface of the brain were imaged by two-photon confocal microscopy. jGCaMP8m expressed in the layer 5 extratelencephalic-projecting (L5ET) neurons were observed in the mouse that was injected AAV-jGCaMP8m-P2A-CyRFP1. The brain surface was perfused with ACSF-HEPES. The mouse was imaged two times at conscutive days. This is the imaging data at the first day. 2 channels are stacked into a single file. 1-501 frames for jGCaMP8m, 502-1002 frames for CyRFP1.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
502.2 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
calcium ion transport
Cellular Component (CC)
cell body
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
1.043 micrometer
Y scale
1.043 micrometer
Z scale
-
T scale
0.169 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project
Source