Detail of Fig5f_odor_SDL
Project
SSBD:Repository
Title
Time-lapse calcium imaging of mouse olfactory bulb in vivo using SeeDB-Live
Description
Odor responses of mitral cells of the olfactory bulb in anesthetized mouse. In the mitral cells, GCaMP6f was expressed. Activities of the mitral cell in response to odor stimulation (1% valeraldehyde) at a depth of 381 micrometer from the surface of the brain were imaged. The odor was delivered to a mouse nose for 5 seconds at 1 litter per minute. The brain surface was perfused with SeeDB-Live.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
24.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
calcium ion transport response to odorant
Cellular Component (CC)
cell body
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.994 micrometer
Y scale
0.994 micrometer
Z scale
-
T scale
1.644 seconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project