Detail of ExtFig10_InVivoVoltage_glomeruli
Project
SSBD:Repository
Title
Time-lapse voltage imaging of glomerular layer of the olfactory bulb in vivo using SeeDB-Live
Description
Odor responses of mitral cells of the olfactory bulb in anesthetized mouse. In the mitral cells, Voltron2 was expressed. Voltage change in the dendrites of the mitral cell in response to odor stimulation (1% amyl acetate) were imaged. The odor was delivered to a mouse nose for 5 seconds at 1 litter per minute. The brain surface was perfused with SeeDB-Live, and imaging was conducted before, during and after application of the odor.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
715.2 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
response to odorant neuronal action potential propagation neuronal action potential back-propagation
Cellular Component (CC)
dendrite
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
2.89 micrometer
Y scale
2.89 micrometer
Z scale
-
T scale
7 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project
Source