Detail of ExtFig8hi_2P_Voltage_L5
Project
SSBD:Repository
Title
Two-photon voltage imaging of JEDI-2P-labelled L5 neurons in S1 of awake mice
Description
Two-photon voltage imaging of JEDI-2P-labelled neurons in layer 5 of somatosensory cortex of awake mice. The brain surface was perfused with SeeDB-Live, and neurons at 564 micrometer depth of the brain was imaged by two-photon confocal microscopy.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
157.4 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
two-photon laser scanning microscopy
(
Fbbi:00000254
)
X scale
0.912 micrometer
Y scale
0.912 micrometer
Z scale
-
T scale
8.8 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project
Source