Detail of Fig6hi_voltageOB_SDL
Project
SSBD:Repository
Title
Time-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 after clearing with SeeDB-Live
Description
Time-lapse images of mitral cells expressing Voltron2 in the olfactory bulb of an anesthetized mouse after clearing with SeeDB-Live. Voltron2 was specifically and sparsely expressed in mitral/tufted cells using the Pcdh21-Cre driver and a Cre-dependent adeno-associated virus (AAV) vector, labeled with JF549-HaloTag ligand (Voltron2_{549}). Glomerular layer of the olfactory bulb (90 micrometer depth of the slice) was imaged.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
89.5 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuronal action potential propagation
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
2.89 micrometer
Y scale
2.89 micrometer
Z scale
-
T scale
7 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project