Detail of ExtFig9abc_SliceVoltage_voltron2
Project
SSBD:Repository
Title
Time-lapse images of mitral cells in the olfactory bulb slice expressing Voltron2
Description
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice at P8. Voltron2, a fast and sensitive chemigenetic voltage indicator, and GCaMP6f were sparsely introduced to mitral/tufted cells by in utero electroporation. The slice in SeeDB-Live was imaged by epifluorescence microscopy. This dataset includes the images of Voltron2_{549}.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
314.2 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuronal action potential propagation neuronal action potential back-propagation
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
2.89 micrometer
Y scale
2.89 micrometer
Z scale
-
T scale
0.5 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project
Source