Detail of Fig6j_voltageOBdendrite_SDL
Project
SSBD:Repository
Title
Time-lapse images of glomerular layer in the olfactory bulb in vivo expressing Voltron2 after clearing with SeeDB-Live
Description
Time-lapse images of mitral cells expressing Voltron2 in the olfactory bulb of an anesthetized mouse after clearing with SeeDB-Live. Voltron2 was specifically and sparsely expressed in mitral/tufted cells using the Pcdh21-Cre driver and a Cre-dependent adeno-associated virus (AAV) vector, labeled with JF549-HaloTag ligand (Voltron2_{549}). Voltage change in the dendrites of the mitral cell in response to odor stimulation (1% amyl acetate) were imaged. The odor was delivered to a mouse nose for 5 seconds at 1 litter per minute.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
715.2 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuronal action potential propagation neuronal action potential back-propagation
Cellular Component (CC)
dendrite
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
2.89 micrometer
Y scale
2.89 micrometer
Z scale
-
T scale
7 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project
Source