Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice using ACSF
Description
Time-lapse images of Voltron2-expressing mitral cells in mouse olfactory bulb slice at P11. Voltron2, a fast and sensitive chemigenetic voltage indicator, was sparsely introduced to mitral/tufted cells by in utero electroporation. Neurons at 150 micrometer depth of the slice in ACSF was imaged by epifluorescence microscopy.
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
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Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology