Detail of ExtFig9def_InVivoVoltage_anesth_ctrl
Project
SSBD:Repository
Title
Time-lapse images of Voltron2-ST labelled L2/3 neurons in S1 of anesthetized mice before clearing with SeeDB-Live
Description
Time-lapse images of Voltron2-ST labelled neurons in layer 2/3 of somatosensory cortex of anesthetized mouse before clearing with SeeDB-Live. Voltron2-ST, a fast and sensitive chemigenetic voltage indicator was introduced to layer 2/3 neurons by in utero electroporation. Cell body of the neurons at 120-150 micrometer depth of the brain was imaged by epifluorescence microscopy. The brain surface was perfused with ACSF-HEPES during imaging.
Release, Updated
2026-08-19
License
CC BY 4.0
Kind
Image data
File Formats
.tif
Data size
1.2 GB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
neuronal action potential propagation
Cellular Component (CC)
cell body dendrite
Biological Imaging Method
fluorescence microscopy
(
Fbbi:00000246
)
X scale
2.89 micrometer
Y scale
2.89 micrometer
Z scale
-
T scale
1 milliseconds
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Inagaki S, Nakagawa-Tamagawa N, Huynh NZ, Kambe Y, Yagasaki R, Manita S, Fujimoto S, Noda T, Mori M, Teranishi A, Takeshima H, Ishikawa K, Naitou Y, Yokoyama T, Sakamoto M, Hayashi K, Kitamura K, Tagawa Y, Okuda S, Sato TK, Imai T. Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo. Nat Methods. 2026 Apr;23(4):839-853.
Related paper(s)
Contact
Shigenori Inagaki, Takeshi Imai
, Kyushu University, Kyushu University
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
, Department of Developmental Neurophysiology, Department of Developmental Neurophysiology
Contributors
OMERO Project
Source