SSBD:database

Key Organism Strain Cell Line Gene Symbol Protein name Protein tag Reporter Project ID Dataset ID Title Description Data Type Gene Ontology Biological Imaging Methods Method Summary Paper Information Medical Subject Headlines Person

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474-Yoshinari-NucleiAutofluoNIR

https://ssbd.riken.jp/database/474-Yoshinari-NucleiAutofluoNIR/

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ssbd-repos-000474

https://ssbd.riken.jp/repository/474/

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Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei

Capturing images of the nuclear dynamics within live cells is an essential technique for comprehending the intricate biological processes inherent to plant cell nuclei. While various methods exist for imaging nuclei, including combining fluorescent proteins and dyes with microscopy, there is a dearth of commercially available dyes for live-cell imaging. In Arabidopsis thaliana, we discovered that nuclei emit autofluorescence in the near-infrared (NIR) range of the spectrum and devised a non-invasive technique for the visualization of live cell nuclei using this inherent NIR autofluorescence. Our studies demonstrated the capability of the NIR imaging technique to visualize the dynamic behavior of nuclei within primary roots, root hairs, and pollen tubes, which are tissues that harbor a limited number of other organelles displaying autofluorescence. We further demonstrated the applicability of NIR autofluorescence imaging in various other tissues by incorporating fluorescence lifetime imaging techniques. Nuclear autofluorescence was also detected across a wide range of plant species, enabling analyses without the need for transformation. The nuclear autofluorescence in the NIR wavelength range was not observed in animal or yeast cells. Genetic analysis revealed that this autofluorescence was caused by the phytochrome protein. Our studies demonstrated that nuclear autofluorescence imaging can be effectively employed not only in model plants but also for studying nuclei in non-model plant species.

2026-06-09

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CC BY 4.0

Image data based on Experiment

7 ( Image datasets: 7, Quantitative data datasets: 0 )

2.6 GB ( Image datasets: 2.6 GB, Quantitative data datasets: 0 bytes )

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Arabidopsis thaliana, Eruca vesicaria subsp. Sativa, Cardamine hirsuta

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cell division, nuclear migration, root development, pollen tube development

nucleus, nuclear envelope nucleoplasm, nucleus root hair, pollen tube

confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246), confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)

600.01 sec, NA, 300.34 sec, 14.90 sec, 20.26 sec, 59.80 sec

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Akira Yoshinari, Reika Isoda, Noriyoshi Yagi, Yoshikatsu Sato, Jelmer J Lindeboom, David W Ehrhardt, Wolf B Frommer, Masayoshi Nakamura (2024) Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei., The Plant journal : for cell and molecular biology

Published in 2024 Mar 20 (Electronic publication in March 20, 2024, midnight )

• doi: 10.1111/tpj.16699
• pmid: 38509728
  

(Abstract) Capturing images of the nuclear dynamics within live cells is an essential technique for comprehending the intricate biological processes inherent to plant cell nuclei. While various methods exist for imaging nuclei, including combining fluorescent proteins and dyes with microscopy, there is a dearth of commercially available dyes for live-cell imaging. In Arabidopsis thaliana, we discovered that nuclei emit autofluorescence in the near-infrared (NIR) range of the spectrum and devised a non-invasive technique for the visualization of live cell nuclei using this inherent NIR autofluorescence. Our studies demonstrated the capability of the NIR imaging technique to visualize the dynamic behavior of nuclei within primary roots, root hairs, and pollen tubes, which are tissues that harbor a limited number of other organelles displaying autofluorescence. We further demonstrated the applicability of NIR autofluorescence imaging in various other tissues by incorporating fluorescence lifetime imaging techniques. Nuclear autofluorescence was also detected across a wide range of plant species, enabling analyses without the need for transformation. The nuclear autofluorescence in the NIR wavelength range was not observed in animal or yeast cells. Genetic analysis revealed that this autofluorescence was caused by the phytochrome protein. Our studies demonstrated that nuclear autofluorescence imaging can be effectively employed not only in model plants but also for studying nuclei in non-model plant species.

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Akira Yoshinari, Masayoshi Nakamura , Nagoya University, Nagoya University , Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules , Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules

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