Detail of Fig2c_NIR_SYBR_Arabi_Pollen_nucleus_WT
Project
SSBD:Repository
Title
The time-lapse NIR autofluorescence and SYBR Green I images of vegetative nucleus during pollen tube elongation in wild-type Arabidopsis
Description
The DNA of wild-type Arabidopsis Col-0 was co-stained with SYBR Green I, a green fluorescent dye, to measure nuclear near-infrared (NIR) autofluorescence in pollen. Time-lapse NIR autofluorescence imaging revealed the dynamics of the vegetative nucleus during pollen tube elongation in wild-type Arabidopsis. The NIR autofluorescence promoted clearer imaging of the vegetative nucleus than DNA dyes during pollen tube elongation.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
czi
Data size
106.9 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
pollen tube development
Cellular Component (CC)
pollen tube
Biological Imaging Method
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
0.198 micrometer
Y scale
0.198 micrometer
Z scale
-
T scale
14.90 sec
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)
Contact
Akira Yoshinari, Masayoshi Nakamura
, Nagoya University, Nagoya University
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
Contributors
OMERO Project
Source