Detail of FigS2_NIR_H2B_Arabi_RootMeristem_EpiderCell_Mut
Project
SSBD:Repository
Title
The time-lapse NIR autofluorescence images of epidermal cells in root meristemetic zone of mutant Arabidopsis expressing H2B-mClover
Description
The nuclear near-infrared (NIR) autofluorescence was observe the epidermal cells in root meristimetic zone of 4-day-old mutant Arabidopsis expressing H2B-mClover. Time-lapse NIR autofluorescence imaging visualized epidermal cells in the root meristematic zone of mutant Arabidopsis. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
czi
Data size
901.5 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
cell division
Cellular Component (CC)
nuclear envelope nucleoplasm
Biological Imaging Method
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
0.312 micrometer
Y scale
0.312 micrometer
Z scale
1.5 micrometer
T scale
600.01 sec
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)
Contact
Akira Yoshinari, Masayoshi Nakamura
, Nagoya University, Nagoya University
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
Contributors
OMERO Project
Source