Detail of Fig2d_NIR_H2B_Arabi_RootHair_nuclei_Mut
Project
SSBD:Repository
Title
The time-lapse NIR autofluorescence images of nuclear migration in mutant Arabidopsis expressing H2B-mClover
Description
The nuclear migration towards the apical or basal side in mutant Arabidopsis root hair cells expressing H2B-mClover, which is a fluorescently tagged version of the histone protain H2B. Time-lapse NIR autofluorescence imaging revealed the nuclear migration in mutant Arabidopsis root hair cells. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
czi
Data size
33.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
nuclear migration
Cellular Component (CC)
nucleus root hair
Biological Imaging Method
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
0.312 micrometer
Y scale
0.312 micrometer
Z scale
-
T scale
300.34 sec
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)
Contact
Akira Yoshinari, Masayoshi Nakamura
, Nagoya University, Nagoya University
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
Contributors
OMERO Project
Source