Detail of Fig2d_NIR_H2B_Arabi_RootHair_nuclei_Mut
Project
SSBD:Repository
Title
The time-lapse NIR autofluorescence images of nuclear migration in mutant Arabidopsis expressing H2B-mClover
Description
The nuclear migration towards the apical or basal side in mutant Arabidopsis root hair cells expressing H2B-mClover, which is a fluorescently tagged version of the histone protain H2B. Time-lapse NIR autofluorescence imaging revealed the nuclear migration in mutant Arabidopsis root hair cells. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
czi
Data size
33.7 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
nuclear migration
Cellular Component (CC)
nucleus root hair
Biological Imaging Method
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
0.312 micrometer
Y scale
0.312 micrometer
Z scale
-
T scale
300.34 sec
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
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Filter set
-
Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)
Akira Yoshinari, Reika Isoda, Noriyoshi Yagi, Yoshikatsu Sato, Jelmer J Lindeboom, David W Ehrhardt, Wolf B Frommer, Masayoshi Nakamura (2024) Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei., The Plant journal : for cell and molecular biology
Published in 2024 Mar 20 (Electronic publication in March 20, 2024, midnight )
- doi: 10.1111/tpj.16699
-
pmid: 38509728
(Abstract) Capturing images of the nuclear dynamics within live cells is an essential technique for comprehending the intricate biological processes inherent to plant cell nuclei. While various methods exist for imaging nuclei, including combining fluorescent proteins and dyes with microscopy, there is a dearth of commercially available dyes for live-cell imaging. In Arabidopsis thaliana, we discovered that nuclei emit autofluorescence in the near-infrared (NIR) range of the spectrum and devised a non-invasive technique for the visualization of live cell nuclei using this inherent NIR autofluorescence. Our studies demonstrated the capability of the NIR imaging technique to visualize the dynamic behavior of nuclei within primary roots, root hairs, and pollen tubes, which are tissues that harbor a limited number of other organelles displaying autofluorescence. We further demonstrated the applicability of NIR autofluorescence imaging in various other tissues by incorporating fluorescence lifetime imaging techniques. Nuclear autofluorescence was also detected across a wide range of plant species, enabling analyses without the need for transformation. The nuclear autofluorescence in the NIR wavelength range was not observed in animal or yeast cells. Genetic analysis revealed that this autofluorescence was caused by the phytochrome protein. Our studies demonstrated that nuclear autofluorescence imaging can be effectively employed not only in model plants but also for studying nuclei in non-model plant species.
Contact
Akira Yoshinari, Masayoshi Nakamura
, Nagoya University, Nagoya University
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
Contributors
OMERO Project
Source