Detail of MovS3_NIR_CardaHir_GrowingRoot_nuclei


Project
SSBD:Repository
Title
The time-lapse NIR autofluorescence imaging of root elongation in Cardamine hirsuta
Description
The nuclear near-infrared (NIR) autofluorescence was observed more than 4 h in root elongation of 4-day-old Cardamine hirsuta. Time-lapse NIR autofluorescence images of root elongation were obtained. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
czi
Data size
601.2 MB

Organism
Cardamine hirsuta ( NCBI:txid50463 )
Strain(s)
-
Cell Line
-

Datatype
-
Molecular Function (MF)
Biological Process (BP)
root development
Cellular Component (CC)
nucleus
Biological Imaging Method
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
1.248 micrometer
Y scale
1.248 micrometer
Z scale
-
T scale
59.80 sec

Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-

Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)

Contact
Akira Yoshinari, Masayoshi Nakamura , Nagoya University, Nagoya University , Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules , Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
Contributors

OMERO Dataset
OMERO Project
Source