The time-lapse NIR autofluorescence imaging of root elongation in Cardamine hirsuta
Description
The nuclear near-infrared (NIR) autofluorescence was observed more than 4 h in root elongation of 4-day-old Cardamine hirsuta. Time-lapse NIR autofluorescence images of root elongation were obtained. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root.
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246) time lapse microscopy (FBbi_00000249)
X scale
1.248 micrometer
Y scale
1.248 micrometer
Z scale
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T scale
59.80 sec
Image Acquisition
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Objective model
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Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)
Contact
Akira Yoshinari, Masayoshi Nakamura
, Nagoya University, Nagoya University
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules