Detail of Fig4c_NIR_Roquette_Root_nuclei
Project
SSBD:Repository
Title
The NIR depth-coding autofluorescence images of nuclei in Roquette roots
Description
A depth-coding image of nuclei based on z-stack images in roquette roots of 4-day-old Eruca vesicaria subsp. Sativa. The NIR autofluorescence imaging approach has no need for a fluorescent protein reporter and can reduce the genetic manipulation time of introducing encoded nuclear markers into mutant plants to improve the efficiency of monitoring the cell nuclei movement in root. Z-stack images were taken at different depths in a specimen and used to observe three dimensional structures.
Release, Updated
2026-06-09
License
CC BY 4.0
Kind
Image data
File Formats
czi
Data size
132.8 MB
Datatype
-
Molecular Function (MF)
Biological Process (BP)
nuclear migration
Cellular Component (CC)
nucleus
Biological Imaging Method
confocal microscopy (FBbi_00000251) fluorescence microscopy (FBbi_00000246)
X scale
0.624 micrometer
Y scale
0.624 micrometer
Z scale
2 micrometer
T scale
-
Image Acquisition
Experiment type
-
Microscope type
-
Acquisition mode
-
Contrast method
-
Microscope model
-
Detector model
-
Objective model
-
Filter set
-
Summary of Methods
Yoshinari A, Isoda R, Yagi N, Sato Y, Lindeboom JJ, Ehrhardt DW, Frommer WB, Nakamura M. Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei. Plant J. 2024 Jun;118(5):1699-1712.
Related paper(s)
Contact
Akira Yoshinari, Masayoshi Nakamura
, Nagoya University, Nagoya University
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
, Institute of Transformative Bio-Molecules, Institute of Transformative Bio-Molecules
Contributors
OMERO Project
Source