SSBD:database v3

409-Ichimura-AMATERAS

Project

Title

Ultra-large FOV imaging by AMATERAS-2

Description

The authors established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, they built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 µm across an FOV of approximately 1.5×1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. They applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. They also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hr, visualizing the movement of over 4.0×105 vascular endothelial cells and quantitatively analyzing their dynamics. Their results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.

License

CC BY

Released at

Oct. 20, 2025, midnight

Updated at

Funding

Grant-in-Aid for Scientific Research on Innovative Areas “Singularity Biology (No. 8007)” 21H00431 (YS), 18H05416 (HH), 18H05412 (SO), and 18H05410, 18H05408 (TN) Grant-in-Aid for Transformative Research Areas (A) “Seeing through Scattering Media (No. 20A207)” 21H05590 and 23H041350 (TI) the Research Program of "Five-star Alliance" in "NJRC Mater. & Dev." (TN) Precursory Research for Embryonic Science and Technology (PRESTO) JPMJPR18G2 (TI) JSPS KAKENHI JP23H00395 and JP24K22022 (HH) AMED Brain/MINDS JP21dm0207117 (HH) and BINDS JP23ama121054 and JP23ama121052 (HH) The Uehara Memorial Foundation (TN) Takeda Science Foundation (TN, HH) Core Research for Evolutionary Science and Technology (CREST) JPMJCR15N3 (TN), JPMJCR1926 (KS, SO) RIKEN Cluster for Science, Technology and Innovation Hub (SO) JST NBDC Grant Number JPMJND2201 (SO)

# of image datasets

7

Papers

Papers

Paper information: Ichimura T, Kakizuka T, Taniguchi Y, Ejima S, Sato Y, Itano K, Seiriki K, Hashimoto H, Sugawara K, Itoga H, Onami S, Nagai T. Volumetric trans-scale imaging of massive quantity of heterogeneous cell populations in centimeter-wide tissue and embryo. Elife. 2025 Feb 3;13:RP93633,
Pubmed ID: 39899352,
PMCID: PMC11790249,
DOI: https://doi.org/10.7554/eLife.93633,
URL: https://pubmed.ncbi.nlm.nih.gov/39899352/

Contacts

Contacts

Taro Ichimura (Contact) , Institute for Open and Transdisciplinary Research Initiatives , Osaka University
Takeharu Nagai (Contact) , Institute for Open and Transdisciplinary Research Initiatives , Osaka University
Taishi Kakizuka (Imaging Contributor) , Institute for Open and Transdisciplinary Research Initiatives , Osaka University
Yoshitsugu Taniguchi (Imaging Contributor) , SIGMAKOKI CO., LTD
Satoshi Ejima (Imaging Contributor) , SIGMAKOKI CO., LTD
Yuki Sato (Imaging Contributor) , Department of Anatomy and Cell Biology , Kyushu University
Kaoru Seiriki (Imaging Contributor) , Graduate School of Pharmaceutical Sciences , Osaka University
Hitoshi Hashimoto (Imaging Contributor) , Institute for Open and Transdisciplinary Research Initiatives , Osaka University

Biosamples

Organisms

Mus musculus (NCBI:txid10090)
Homo sapiens (NCBI:txid9606)
Coturnix japonica (NCBI:txid93934)

Strains

Tg (tie1:H2B-eYFP)
C57BL/6J

Cells

endothelial cell of vascular tree (CL_0002139)

pluripotent stem cell (CL_0002248)

Cell lines

hiPSC line 201B7

Ontologies

UBERON

vascular system (UBERON_0007798)
embryo (UBERON_0000922)
brain (UBERON_0000955)