SSBD:database

Project ID

409-Ichimura-AMATERAS

Project SID

409

Title

Ultra-large FOV imaging by AMATERAS-2

Description

The authors established a volumetric trans-scale imaging system with an ultra-large field-of-view (FOV) that enables simultaneous observation of millions of cellular dynamics in centimeter-wide three-dimensional (3D) tissues and embryos. Using a custom-made giant lens system with a magnification of ×2 and a numerical aperture (NA) of 0.25, and a CMOS camera with more than 100 megapixels, they built a trans-scale scope AMATERAS-2, and realized fluorescence imaging with a transverse spatial resolution of approximately 1.1 µm across an FOV of approximately 1.5×1.0 cm2. The 3D resolving capability was realized through a combination of optical and computational sectioning techniques tailored for our low-power imaging system. They applied the imaging technique to 1.2 cm-wide section of mouse brain, and successfully observed various regions of the brain with sub-cellular resolution in a single FOV. They also performed time-lapse imaging of a 1-cm-wide vascular network during quail embryo development for over 24 hr, visualizing the movement of over 4.0×105 vascular endothelial cells and quantitatively analyzing their dynamics. Their results demonstrate the potential of this technique in accelerating production of comprehensive reference maps of all cells in organisms and tissues, which contributes to understanding developmental processes, brain functions, and pathogenesis of disease, as well as high-throughput quality check of tissues used for transplantation medicine.

Submission Date

2024-12-17

Opened Date

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Release Date

2025-11-26

Update Date

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Kind

License

Project URL

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Project DOI

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Metadata Version

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Template Version

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Funding Information

Grant-in-Aid for Scientific Research on Innovative Areas “Singularity Biology (No. 8007)” 21H00431 (YS), 18H05416 (HH), 18H05412 (SO), and 18H05410, 18H05408 (TN) Grant-in-Aid for Transformative Research Areas (A) “Seeing through Scattering Media (No. 20A207)” 21H05590 and 23H041350 (TI) the Research Program of "Five-star Alliance" in "NJRC Mater. & Dev." (TN) Precursory Research for Embryonic Science and Technology (PRESTO) JPMJPR18G2 (TI) JSPS KAKENHI JP23H00395 and JP24K22022 (HH) AMED Brain/MINDS JP21dm0207117 (HH) and BINDS JP23ama121054 and JP23ama121052 (HH) The Uehara Memorial Foundation (TN) Takeda Science Foundation (TN, HH) Core Research for Evolutionary Science and Technology (CREST) JPMJCR15N3 (TN), JPMJCR1926 (KS, SO) RIKEN Cluster for Science, Technology and Innovation Hub (SO) JST NBDC Grant Number JPMJND2201 (SO)

Organism

Strain

Cell

Cell line

MeSH

Anatomical Entity

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UBERON

Biological Process

Cellular Component

Molecular Function

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Medical Subject Headings

Method involved in biological imaging